Potential uses of milk epithelial cells: a review
Potential uses of milk epithelial cells: a review
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DOI：
10.1051/rnd:2002013
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发表时间：
2002-03-01
期刊：
影响因子：
--
通讯作者：
Jammes, H
中科院分区：
文献类型：
--
作者：
Boutinaud, M;Jammes, H
从不同种属的乳腺收集的分泌物含有异质细胞群，包括不同种属的淋巴细胞、中性粒细胞、巨噬细胞和上皮细胞。影响乳中体细胞数量和细胞类型分布的因素有多种，如种属、感染状况、生理状况和管理措施。上皮细胞在泌乳过程中脱落进入乳汁。它们中的大多数是活的，并表现出完全分化的肺泡细胞的特征。从人、狒狒、牛和山羊的初乳和乳汁中获得的上皮细胞的原代培养物，以及从人和山羊乳汁中建立的细胞系，为研究乳汁生成、免疫传递、癌症研究和病毒感染提供了良好的模型。从乳细胞中提取的RNA已被证明是乳腺中基因表达的代表，因此为基因表达和环境相互作用的分子研究提供了材料来源。
Secretions collected from the mammary gland of different species contain heterogeneous populations of cells including lymphocytes, neutrophils, macrophages and epithelial cells in different species. Several factors influence the somatic cell count in milk and the distribution of cell types, such as species, infection status, physiological status and management practices. The epithelial cells are shed into milk during the lactation process. Most of them are viable and exhibit the characteristics of fully differentiated alveolar cells. Primary cultures of epithelial cells from colostrum and milk of humans, baboons, cows and goats together with established cell lines from human and goat milk, provide a good model for the study of lactogenesis, immunity transmission, cancer research and infection by viruses. The RNA extracted from milk cells have been shown to be representative of gene expression in the mammary gland and thus provide a source of material for molecular studies of gene expression and environmental interactions.