Stabilised Biosensing Using Needle-Based Recess Electrodes
Stabilised Biosensing Using Needle-Based Recess Electrodes
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DOI：
10.1002/elan.201100511
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发表时间：
2012-03-01
期刊：
影响因子：
3
通讯作者：
Vadgama, Pankaj
中科院分区：
文献类型：
--
作者：
Anastasova, Salzitsa;Spehar-Deleze, Anna-Maria;Vadgama, Pankaj
本文报道了一种用于氧和葡萄糖安培监测的凹槽圆盘电极。传感器的基本设计是一个针结构，易于植入，体现了一个80 μ m内径的工作电极的填充金的二氧化硅毛细管。比较了置于不锈钢管内的凹槽和镶嵌圆盘电极（用作反电极/参比电极）。用不同的处理和屏障膜对工作电极表面进行改性，以实现对感兴趣的分析物的选择性。基本针形式是体内使用的理想选择，并且该形式易于扩展到其他分析物靶标。该传感器显示线性工作范围为氧气高达160毫米汞柱的氧分压，和葡萄糖从1至10毫米。生物污垢，暴露于牛血清白蛋白评估，显着减少。凹槽结构的响应时间增加，但仍在生理监测的可接受范围内。的传感器的操作稳定性证明，以及在生理相关水平的抗坏血酸，尿酸，对乙酰氨基酚和邻苯二酚的存在下，过氧化物的无干扰检测。初步的体内试验显示出对葡萄糖的良好反应。
A recess disk electrode for amperometric monitoring of oxygen and glucose is reported. The basic design of sensor is a needle structure for easy implantation, embodying a gold filled silica capillary with a 80 mu m inner diameter working electrode. Recess and inlaid disc electrodes, placed inside stainless steel tubes which served as a counter/reference electrode, were compared. The working electrode surface was modified with different treatments and barrier membranes to achieve selectivity for the analytes of interest. The basic needle format is ideal for in vivo use, and the format is easily extendable to other analyte targets. The sensors show linear working range for oxygen up to 160 mmHg partial pressure of oxygen, and for glucose from 1 to 10 mM. Bio-fouling, as assessed by exposure to bovine serum albumin, was significantly reduced. Response times for the recess construct was increased but remained within the acceptable range for physiological monitoring. The operational stability of the sensors is demonstrated as well as the interference-free detection of peroxide in the presence of physiologically relevant levels of ascorbic acid, uric acid, acetaminophen, and catechol. Preliminary in vivo tests showed excellent response towards glucose.