A polymorphic DNA marker on chromosome 10 linked to RBP3 on the MEN2A side.
A polymorphic DNA marker on chromosome 10 linked to RBP3 on the MEN2A side.
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10 号染色体上的多态性 DNA 标记与 MEN2A 侧的 RBP3 相连。
DOI：
10.1159/000132639
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Kidd,KK
中科院分区：
文献类型：
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作者：
Wu,J;Cavenee,WK;Miki,T;Kidd,KK
许多由功能基因或任意DNA序列表示的多态基因座已整合到人类10号染色体的遗传图谱中(Donis-Keller等人)。1987年；Farrer et al.1988年：Lathrop ct al..1988年：三木等人..1988年：迈尔斯等人..1988年；Wu et al.1988a，b)。多发性内分泌腺瘤，2A型(MEN2A型)的基因也被分配到该染色体的着丝粒周围区域(Mathew等人)。1987年；辛普森等人，1987年)。然而，这些相邻基因座之间分辨率小于30厘米的地图覆盖的区域仅限于RBP3基因座的长臂一侧，即。远离MEN2A区域的一侧。我们报道了由匿名探针p7A9定义的限制性片段长度多态(RFLP)的连锁图谱ping。利用多点分析和配对分析，该RFLP(现在的D10S24基因座)被定位到10号染色体上。从RBP3基因座到短臂一侧约有17个重组单位。P7A9是一个含有6.5kb人类序列的重组载体，克隆到载体pBR322的Dill位点。与核酸内切酶消化的人基因组DNA的Southern杂交表明，该插入片段含有人的重复序列(S)。由p7A9定义的RTLP基因座有一个被检查过的过去。根据非常早期的数据，它最初被认为位于12号染色体上，并被赋予了符号D12S3(Skolnick等人。1983年)。1985年在IIGM8上。一些人已经知道，这个RFLP不在12号染色体上，在Willard等人(1985)的RFLP表中被列为未分配，但Grzeschik和Kazazian(1985)仍将其暂时列为12号染色体上的。由于一直没有发表，在HGM9，Kazazian等人(1987)继续将其列为12号染色体上的临时染色体，为了一致，Pearson等人(1987)的RFLP表也将其列为D12S3。不幸的是，已知它不在12号染色体上的警告被省略了。关于它的RFLP系统的错误也一直存在：自1983年以来列出的Taq1和MSPL RFLP的条带大小已经被认为是不准确的：它们是非常粗略的初步估计，但从来没有人发表过更准确的估计。由于这个基因座可能位于染色体10上，我们对这个克隆进行了详细的RFLP研究。从p7A9克隆的pSTH5.V/N1双拷贝片段中分离到一个单拷贝片段。该片段全长约1.3kb，可鉴定出与整个克隆相同的MSPL和Taq1 RFLP。去你的。1显示了这个1.3 kb的片段所揭示的多态模式；这些等位基因的Mendclan遗传是
A number of polymorphic loci, represented either by function al genes or by arbitrary DNA sequences, have been integrated into the genetic map of human chromosome 10 (Donis-Keller et al.. 1987; Farrer et al.. 1988: Lathrop ct al.. 1988: Miki et al.. 1988: Myers et al.. 1988; Wu et al.. 1988a, b). The gene for multiple endocrine neoplasia, type 2A (MEN2A) has also been assigned to the pericentric region of this chromosome (Mathew et al.. 1987; Simpson et al., 1987). The regions covered by these maps with resolution finer than 30 cM between adjacent loci, however, are limited to the long arm side of the RBP3 locus, ie. the side that is away from the MEN2A region. We here report the linkage map ping of the restriction fragment length polymorphism (RFLP) de fined by the anonymous probe p7A9. Using multipoint as well as pairwise analyses, this RFLP (now the D10S24 locus) maps to chromosome 10. about 17 recombination units from the RBP3 locus on the short arm side. p7A9 is a recombinant plasmid containing a 6.5-kb human sequence cloned into the///«dill site of the vector pBR322. The insert contains human repetitive sequence (s) as indicated by its hybridization to Southern blots of endonucleasedigested human genomic DNA. The RTLP locus defined by p7A9 has a check ered past. It was initially thought to be on chromosome 12 based on very pre liminary data and given the symbol D12S3 (Skolnick et al.. 1983). At IIGM8 in 1985. it was known to some that this RFLP was not on chromosome 12 and it was listed as unassigned in the RFLP table of Willard et al.(1985) but still listed as provisionally on chromosome 12 by Grzeschik and Kazazian (1985). Since nothing had ever been published, at HGM9 Kazazian et al.(1987) continued to list it as provisionally on chromosome 12 and the RFLP table of Pearson et al.(1987) also listed it under D12S3 in order to be consistent. Unfortunately, the caveat that it was known not to be on chromosome 12 was omitted. Errors regarding its RFLP systems have also persisted: the band sizes listed since 1983 for the Taql and Mspl RFLPs have been known for some time to be inaccurate: they were preliminary, very rough estimates, but no one has ever published more accurate estimates.Motivated by the possibility that this locus was on chro mosome 10, we undertook a detailed RFLP study of this clone. A single-copy fragment was isolated from the Psth 5. V/N1 double di gest of the p7A9 clone. This fragment is about 1.3 kb and was demonstrated to identify the same Mspl and Taql RFLPs as the whole clone. F ig. 1shows the polymorphic pattern revealed by this 1.3-kb fragment; the Mendclian inheritance of these alleles was