What's it like inside prokaryotic cells?
What's it like inside prokaryotic cells?
复制标题
原核细胞内部是什么样的?
DOI:
10.1099/13500872-140-2-213
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发表时间:
1994
期刊:
影响因子:
--
通讯作者:
M. Kogut
中科院分区:
文献类型:
--
作者:
M. Kogut
! The worst of it is that there are no ‘controls’! microscopy (4). Sizes were usually gven in! for such adventitious changes in cell pellets-! megadaltons W a). Since that time the sizes! only replication and consistency of results.! of some plasmids have been revised in the light! I would appeal to all interested in the ‘true of mapping studies involving restriction i intracellular environment’of micro-organisms! fragment analysis of whole plasmids and the: to come to grips with this problem and obtain sizes are frequently given in hlobase pairs (kb). i valid, repeated and consistent values for For example, the size of RP4 was shown to be! intracellular volumes (1, 2). It is worth the! larger (approx. 60 kb) than previously! effort if we want to understand intracellular! determined (3).! conditions and events. i In studying plasmids present in plant: Margot Kogut pathogenic bacteria, I recently obtained a strain! Life Sciences Division, King‘s College, i of Escherichia cob K12 (39R861), carrying four i Kensington, London W8 7AH, UK. plasmids which are useful as size standards. Tel:+ 44 71 333 4358. Fax+ 44 71 333 4500. These plasmids, XY S-2 and NTP168 are i 98, 42, 23.9 and 4-6 MDa, respectively, and this