ACTIVATION OF PROTO-ONCOGENES - AN IMMEDIATE EARLY EVENT IN HUMAN CYTOMEGALOVIRUS-INFECTION
ACTIVATION OF PROTO-ONCOGENES - AN IMMEDIATE EARLY EVENT IN HUMAN CYTOMEGALOVIRUS-INFECTION
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DOI：
10.1126/science.1689075
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发表时间：
1990-02-02
期刊：
影响因子：
56.9
通讯作者：
ALBRECHT, T
中科院分区：
文献类型：
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作者：
BOLDOGH, I;ABUBAKAR, S;ALBRECHT, T
人巨细胞病毒感染后，原癌基因c-fos、c-jun和c-myc的RNA水平迅速升高。紫外线照射(加或不加补骨脂素)灭活病毒感染性，或用放线菌素或山奈素抑制翻译，都不会对原癌基因的增强表达产生不利影响，即使这些治疗大大减少或消除了对早期病毒抗原的即刻检测。在α-Amanitin或放线菌素D的存在下，原癌基因的RNA水平的增加被阻止。因此，这些癌基因的表达似乎是由病毒蛋白质合成开始之前发生的事件诱导的，可能是通过病毒颗粒与细胞表面的相互作用。
A rapid increase in the RNA levels of the proto-oncogenes c-fos, c-jun, and c-myc was detected after human cytomegalvirus infection. Neither inactivation of viral infectivity with ultraviolet irradiation (with or without psoralen), nor inhibition of translation with cycloheximide or anisomycin adversely affected the enhanced expression of proto-oncogenes, even though these treatments substantially reduced or eliminated the detection of immediate early viral antigens. The increase in the RNA levels of the proto-oncogenes was prevented in the presence of .alpha.-amanitin or actinomycin D. Thus, expression of these oncogenes appears to be induced by events occurring before the onset of viral protein synthesis, perhaps by the interaction of viral particles with the cell surface.