Identification of elicitor-responsive proteins in rice leaves by a proteomic approach
Identification of elicitor-responsive proteins in rice leaves by a proteomic approach
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通过蛋白质组学方法鉴定水稻叶片中的激发子响应蛋白
DOI：
10.1002/pmic.200800192
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发表时间：
2009-05-01
期刊：
影响因子：
3.4
通讯作者：
Wang, Zhenzhong
中科院分区：
文献类型：
--
作者：
Liao, Ming;Li, Yunfeng;Wang, Zhenzhong
进行实验以鉴定用从稻瘟病真菌 Magnaporthe grisea 的一个小种 ZC(13) 中纯化的糖蛋白引发子 CSB I 处理后的水稻 (Oryza sativa L.) 植物中的差异表达蛋白。水稻的两个近等基因系 C101A51 和 CO39 与 ZC(13) 的相互作用分别导致完全不亲和和亲和类型。在用 CSB I 处理后 12 和 24 小时从水稻叶片中提取蛋白质。使用 2-DE 检查总蛋白质的时间变化。在每张凝胶上可重复检测到的 900 多个蛋白点中，至少在一个时间点内有 11 个上调，3 个下调，7 个新诱导。通过线性离子阱四极杆 (LTQ)-MS/MS 鉴定了 21 个差异表达蛋白。根据报告的假定功能，将鉴定出的蛋白质分为六类：(i) 防御蛋白（PR-10a、PR-5 和假定的盐诱导蛋白），(ii) 信号转导（核苷二磷酸激酶和假定的 profilin），(iii) ROS（Mn-SOD、Cu/Zn-SOD、GST 和 CAT），(iv) 程序性细胞死亡（翻译控制的肿瘤） (v) 分子生物合成（假定的核糖体蛋白 S5、假定的核糖体蛋白 L12、假定的翻译延伸因子 Tu 和假定的伴侣蛋白 21 前体）和 (vi) 代谢（假定的果糖二磷酸醛缩酶 I 类、假定的苹果酸脱氢酶、胞质苹果酸脱氢酶、假定的酸 磷酸酶、假定的转酮醇酶 1 和 γ 羟基丁酸脱氢酶样蛋白）。所有这些蛋白质（除了 Cu/Zn-SCD、推定的酸性磷酸酶和翻译控制的肿瘤蛋白）在 C101A51 中的诱导速度比 CO39 中的诱导速度更快且程度更高。这些数据表明，不相容的水稻品系可能拥有更灵敏的识别系统，能够以更有效的方式识别特定的化学、生物或物理触发因素并做出反应，从而引发早期和快速的防御反应。
Experiments were conducted to identify the differentially expressed proteins in rice (Oryza sativa L.) plants after treatment with the glycoprotein elicitor CSB I, purified from ZC(13), a race of the rice blast fungus Magnaporthe grisea. The interactions of two near isogenic lines of rice, C101A51 and CO39, with ZC(13) resulted in completely incompatible and compatible types, respectively. Proteins were extracted from rice leaves at 12 and 24 h after treatment with CSB I. Temporal changes in total proteins were examined using 2-DE. Among more than 900 protein spots reproducibly detected on each gel, 11 were up-regulated, three were down-regulated and seven were newly induced during, at a minimum, one time point. Twenty-one differentially expressed proteins were identified by linear ion trap quadrupole (LTQ)-MS/MS. The identified proteins were classified into six categories based on their putative function reported: (i) defense proteins (PR-10a, PR-5 and putative salt-induced protein), (ii) signal transduction (nucleoside diphosphate kinase and putative profilin), (iii) ROS (Mn-SOD, Cu/Zn-SOD, GST and CAT), (iv) programmed cell death (translationally controlled tumor protein), (v) molecule biosynthesis (putative ribosomal protein S5, putative ribosomal protein L12, putative translational elongation factor Tu and putative chaperonin 21 precursor) and (vi) metabolism (putative fructose-bisphosphate aldolase class-I, putative malate dehydrogenase, cytoplasmic malate dehydrogenase, putative acid phosphatase, putative transketolase1 and gamma hydroxybutyrate dehydrogenase-like protein). All of these proteins (except Cu/Zn-SCD, putative acid phosphatase and translationally controlled tumor protein) were induced faster and to a higher degree in C101A51 than in CO39. These data suggest that the incompatible rice line may possess a more sensitive recognition system that can identify and react to specific chemical, biological or physical triggers in a more efficient manner, thus eliciting an early and fast defense response.