Halorubrum chaoviator Mancinelli et al. 2009 is a later, heterotypic synonym of Halorubrum ezzemoulense Kharroub et al. 2006. Emended description of Halorubrum ezzemoulense Kharroub et al. 2006
Halorubrum chaoviator Mancinelli et al. 2009 is a later, heterotypic synonym of Halorubrum ezzemoulense Kharroub et al. 2006. Emended description of Halorubrum ezzemoulense Kharroub et al. 2006
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DOI：
10.1099/ijsem.0.003005
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发表时间：
2018-11-01
影响因子：
2.8
通讯作者：
Ventosa, Antonio
中科院分区：
文献类型：
--
作者：
Corral, Paulina;de la Haba, Rafael R.;Ventosa, Antonio
对伊泽穆尔盐红菌（Halorubrum ezzemoulense）Kharroub et al. 2006、Chaoviator Mancinelli et al. 2009以及与这些盐古菌相关的8个新盐红菌菌株进行了多相比较分类学研究。利用atpB、EF-2、glnA、ppsA和rpoB这5个连锁管家基因进行多位点序列分析，并基于从其基因组中获得的757个核心蛋白序列进行系统发育分析，结果表明Hrr. Ezzemoulense DSM 17463（T），Hrr. Chaoviator Halo-G *（T）（= DSM 19316（T））和八个Halorubrum菌株形成了一个强大的簇，与Halorubrum属的其余物种明显分开。通过基因组间距离计算器（GGDC）计算的orthoANI值和数字DNA-DNA杂交值显示了Hrr之间的百分比。Ezzemoulense DSM 17463（T），Hrr. ChaoviatorDSM 19316（T）和Halorubrum 8个菌株的分离率分别为99.4 - 97.9%和95.0 - 74.2%，而这些菌株和Halorubrum最接近种的模式菌株的分离率分别为88.7 - 77.4%和36.1 - 22.3%。虽然观察到一些差异，表型和极性脂质配置文件是非常相似的所有研究菌株。总的来说，这些数据表明，HRR。埃泽穆伦塞山Chaoviator和8个新的Halorubrum分离物构成一个单一的物种。因此，HRR。chaoviator应该被认为是Hrr的一个较晚的异型同义词。伊泽穆伦我们提出了一个修订的描述HRR。ezzemoulense，包括HRR的功能。Chaoviator和八个新分离物的那些。
A polyphasic comparative taxonomic study of Halorubrum ezzemoulense Kharroub et al. 2006, Halorubrum chaoviator Mancinelli et al. 2009 and eight new Halorubrum strains related to these haloarchaeal species was carried out. Multilocus sequence analysis using the five concatenated housekeeping genes atpB, EF-2, glnA, ppsA and rpoB, and phylogenetic analysis based on the 757 core protein sequences obtained from their genomes showed that Hrr. ezzemoulense DSM 17463(T), Hrr. chaoviator Halo-G*(T) (=DSM 19316(T)) and the eight Halorubrum strains formed a robust cluster, clearly separated from the remaining species of the genus Halorubrum. The orthoANI value and digital DNA-DNA hybridization value, calculated by the Genome-to-Genome Distance Calculator (GGDC), showed percentages among Hrr. ezzemoulense DSM 17463(T), Hrr. chaoviator DSM 19316(T) and the eight Halorubrum strains ranging from 99.4 to 97.9 %, and from 95.0 to 74.2 %, respectively, while these values for those strains and the type strains of the most closely related species of Halorubrum were 88.7-77.4% and 36.122.3 %, respectively. Although some differences were observed, the phenotypic and polar lipid profiles were quite similar for all the strains studied. Overall, these data show that Hrr. ezzemoulense, Hrr. chaoviator and the eight new Halorubrum isolates constitute a single species. Thus, Hrr. chaoviator should be considered as a later, heterotypic synonym of Hrr. ezzemoulense. We propose an emended description of Hrr. ezzemoulense, including the features of Hrr. chaoviator and those of the eight new isolates.