High strength biocompatible PEG single-network hydrogels
High strength biocompatible PEG single-network hydrogels
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DOI：
10.1039/c4ra01870b
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发表时间：
2014-06
期刊：
影响因子：
3.9
通讯作者：
Shanshan Qian;Chao Zhou;Liqun Xu;F. Yao;L. Cen;G. Fu
中科院分区：
文献类型：
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作者：
Shanshan Qian;Chao Zhou;Liqun Xu;F. Yao;L. Cen;G. Fu
本工作通过对聚合物网络分子拓扑结构的精确设计和控制，成功制备了具有极高强度的聚乙二醇基单链水凝胶。首先，通过α，ω-二环氧PEG与炔丙胺的胺-环氧扩链反应，合成了主链上具有均匀分散的炔基侧基的PEG大分子（PEGn（CCH））m。（PEGn（CCH））m和α，ω-二叠氮基PEGn（PEGn（N3）2）的叠氮-炔1，3-偶极环加成反应（CuAAC）得到坚韧PEG基水凝胶（Gel-PEGn（N3）2-（PEGn（CCH））m）。凝胶-PEGn（N3）2-（PEGn（CCH））m网络的晶格尺寸可以通过改变（PEGn（CCH））m和PEGn（N3）2中PEG重复片段的链长来定制。与传统的由CuAAC制备的PEG水凝胶（如由四（2-丙炔氧基甲基）甲烷和PEGn（N3）2制备的水凝胶）不同，目前的新型水凝胶不仅具有高达62.5 MPa的高机械强度，而且由于（PEGn（CCH））m大分子中存在三乙胺基团而有利于生物降解。此外，根据体外细胞毒性试验，证明了Gel-PEGn（N3）2-（PEGn（CCH））m具有优异的生物相容性。因此，可以确定Gel-PEGn（N3）2-（PEGn（CCH））m具有用于人造医疗装置或再生医学支架材料的有希望的潜力。
In this work, PEG-based single-chain hydrogels with extremely high strength were successfully prepared via precise design and control over the molecular topology of the polymeric network. Initially, well-defined PEG macromolecules with uniformly dispersed pendant alkynyl groups (PEGn(CCH))m on their main chains were synthesized via amine-epoxy chain extension reaction of α,ω-diepoxy PEG and propargylamine. The subsequent copper(I)-catalyzed azide–alkyne 1,3-dipolar cycloaddition (CuAAC) of (PEGn(CCH))m and α,ω-diazido PEGn (PEGn(N3)2) gave rise to tough PEG-based hydrogels (Gel-PEGn(N3)2-(PEGn(CCH))m). The lattice size of the Gel-PEGn(N3)2-(PEGn(CCH))m networks can be tailored by varying chain lengths of PEG repeating segments in (PEGn(CCH))m and PEGn(N3)2. Different from traditional PEG hydrogels prepared by CuAAC, such as hydrogels from tetrakis(2-propynyloxymethyl)methane and PEGn(N3)2, the current novel hydrogels possess not only a high mechanical strength up to 62.5 MPa, but are also biodegradable favored by the presence of triethylamine groups in the (PEGn(CCH))m macromolecules. Furthermore, excellent biocompatibility of the Gel-PEGn(N3)2-(PEGn(CCH))m was demonstrated according to the in vitro cytotoxicity assay. Hence, it can be ascertained that Gel-PEGn(N3)2-(PEGn(CCH))m has promising potential for artificial medical devices or scaffolding materials for regenerative medicine.