Influence of the parental virus strain on the virulence and immunogenicity of recombinant vaccinia viruses expressing HBV preS2-S protein or VZV glycoprotein I
Influence of the parental virus strain on the virulence and immunogenicity of recombinant vaccinia viruses expressing HBV preS2-S protein or VZV glycoprotein I
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DOI：
10.1016/0264-410x(96)00008-4
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发表时间：
1996-08-01
期刊：
影响因子：
5.5
通讯作者：
Vonka, V
中科院分区：
文献类型：
--
作者：
Kutinova, L;Ludvikova, V;Vonka, V
利用从天花Sevac VARIE疫苗（Praha株）获得的5株三斑块纯化痘苗病毒（VV）和从Wyeth DRYVAX疫苗获得的3株类似的VV病毒株，制备了表达乙型肝炎病毒preS2-S基因的重组体。利用相同的5个普拉哈衍生病毒系构建表达水痘-带状疱疹病毒（VZV）糖蛋白I （gpI）基因的重组体。对重组病毒及其亲本病毒在小鼠体内的残余神经毒力进行了检测。病毒系及其衍生的重组体在这方面有显著不同。小鼠免疫仅在来自相对强毒病毒的重组体中产生高水平的抗hbsag抗体。相比之下，小鼠体内的VZVgpI抗体水平与所有VV-VZV重组体相似，而与亲代病毒系的毒力无关。版权所有爱思唯尔科学有限公司
Five triple-plaque purified vaccinia virus (VV) lines generated from smallpox Sevac VARIE vaccine (strain Praha) and three VV virus lines similarly derived from Wyeth DRYVAX vaccine were used for preparation of recombinants expressing the hepatitis B virus preS2-S gene. The same five Praha-derived virus lines were used to construct recombinants expressing the varicella-zoster virus (VZV) glycoprotein I (gpI) gene. Recombinants and their parental viruses were tested for the residual neurovirulence in mice. The virus lines and the recombinants derived therefrom differed markedly in this respect. Immunization of mice resulted in high levels of anti-HBsAg antibodies only in the case of recombinants derived from the relatively virulent viruses. In contrast, the levels of VZVgpI antibodies in mice were similar with all VV-VZV recombinants irrespective of the virulence of the parental virus line. Copyright (C) 1996 Elsevier Science Ltd.