Distribution and developmental expression of the nerve terminal protein NT75 in the rat cerebellum.
Distribution and developmental expression of the nerve terminal protein NT75 in the rat cerebellum.
复制标题
神经末梢蛋白NT75在大鼠小脑中的分布和发育表达。
DOI：
10.1002/cne.903040403
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Coulter,JD
中科院分区：
文献类型：
--
作者：
Wright,DJ;Ritchie,TC;Coulter,JD
以前的研究表明，在发育中的脊髓神经末梢蛋白NT 75之间的关联NT 75免疫反应性的外观和突触发生的过程。为了进一步研究NT 75表达的时间过程，目前的研究。比较NT 75和突触囊泡蛋白synaptophysin在成年和发育中大鼠小脑和小脑组织培养中的定位。在成人小脑中，密集的NT 75染色仅限于分子层，在那里它与小脑颗粒细胞的平行纤维末梢相关。在发育过程中，NT 75免疫反应性是第一次检测到在小脑皮质作为一个密集的染色带在最深的部分的分子层在出生后第10天。染色区逐渐扩大，占据分子层的更大部分，直到出生后20天左右。突触素染色发生在颗粒细胞过程早于NT 75，并发现整个分子层出生后7天。在数量上，NT 75和突触体素的快速增加发生在出生后的前三周，NT 75活性在出生后第20至30天达到超过成人值50%的水平，而突触体素在出生后第20天达到接近成人水平的平台。在小脑培养物中，神经突中的NT 75染色在几天内发展，与突触接触的发展同时增加，而突触素染色在体外仅1天后就已经存在于大多数神经突中。结果表明，NT 75在发育中的小脑颗粒细胞神经末梢的表达与成熟神经末梢的出现密切相关，这表明该蛋白可能在突触末梢的形成/稳定或突触传递机制中起作用。
Previous studies of the nerve terminal protein NT75 in the developing spinal cord have suggested an association between the appearance of NT75 immunoreactivity and the process of synaptogenesis. To examine the time course of NT75 expression further, the current study. Compared the localization of NT75 and the synaptic vesicle protein synaptophysin in the adult and developing rat cerebellum and in cerebellar tissue cultures. In the adult cerebellum, dense NT75 staining is confined to the molecular layer, where it is associated with parallel fiber endings of cerebellar granule cells. During development, NT75 immunoreactivity is first detectable in the cerebellar cortex as a dense band of staining in the deepest portion of the molecular layer at postnatal day 10. The stained zone expands to occupy a progressively greater portion of the molecular layer until about postnatal day 20. Synaptophysin staining occurs in granule cell processes earlier than NT75 and is found throughout the molecular layer by postnatal day 7. Quantitatively, rapid increases in both NT75 and synaptophysin occur in the first three postnatal weeks, with NT75 activity reaching levels exceeding the adult value by 50% over postnatal days 20 through 30, whereas synaptophysin plateaus at near adult levels by postnatal day 20. In cerebellar cultures, NT75 staining in neurites develops over several days, increasing coincidentally with development of synaptic contacts, whereas synaptophysin staining is already present in most neurites after only 1 day in vitro. The results indicate that NT75 expression in developing cerebellar granule cell nerve terminals is closely associated with the appearance of mature nerve terminals, suggesting that the protein may have a role in the formation/stabilization of the synaptic ending or in the mechanisms of synaptic transmission.