Induction of nitroblue tetrazolium reduction in mouse peritoneal macrophages by tumour promoters and inhibition of the induced nitroblue tetrazolium reduction by some inhibitors.
Induction of nitroblue tetrazolium reduction in mouse peritoneal macrophages by tumour promoters and inhibition of the induced nitroblue tetrazolium reduction by some inhibitors.
复制标题
肿瘤促进剂诱导小鼠腹腔巨噬细胞中硝基蓝四唑还原，并通过某些抑制剂抑制诱导的硝基蓝四唑还原。
DOI：
10.1016/0304-3835(85)90183-1
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发表时间：
1985
期刊：
影响因子：
9.7
通讯作者：
Inui,N
中科院分区：
文献类型：
--
作者：
Ohkawa,Y;Iwata,K;Shibuya,H;Inui,N
Aplysiatoxin和Debromoaplysiatoxin两种聚乙酸酯，以及12-O-十四酰佛波醇-13-乙酸酯(TPA)、甲草醚和硬杀菌素在体外均能促进小鼠腹腔巨噬细胞对NBT的还原。5种TPA类促癌剂对NBT还原的ED50值分别为：TPA 4.2 ng/ml、甲氧西林36 ng/ml、杀线菌素0.53 ng/ml、脱毒毒素1.5 ng/ml、脱溴青霉毒素108 ng/ml。两种促癌抑制剂维甲酸和二溴苯乙酮可抑制这5种促癌剂诱导的NBT还原。讨论了TPA型促癌剂促进肿瘤的可能性，包括细胞膜释放超氧阴离子自由基等类似的机制。
Two polyacetates, aplysiatoxin and debromoaplysiatoxin, as well as 12-O-tetradecanoylphorbol-13-acetate (TPA), mezerein and teleocidin enhance nitroblue tetrazolium (NBT) reduction in mouse peritoneal macrophages in vitro. The ED50values for NBT reduction of these 5 TPA-type tumor promoters were 4.2 ng/ml for TPA, 36 ng/ml for mezerein, 0.53 ng/ml for teleocidin, 1.5 ng/ml for aplysiatoxin and 108 ng/ml for debromo-aplysiatoxin. The NBT reduction induced by the 5 tumor promoters is inhibited by 2 inhibitors of tumor promotion, retinoic acid and dibromoacetophenone. The possibility that tumor promotion by TPA-type tumor promoters involves similar mechanisms such as superoxide anion radicals release in cell membranes is discussed.