A comparison of inflammatory mediators released by basophils of asthmatic and control subjects in response to high-affinity IgE receptor aggregation
A comparison of inflammatory mediators released by basophils of asthmatic and control subjects in response to high-affinity IgE receptor aggregation
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DOI：
10.1159/000109287
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发表时间：
2008-01-01
影响因子：
2.8
通讯作者：
Oliver, Janet M.
中科院分区：
文献类型：
--
作者：
Gilmartin, Laura;Tarleton, Christy A.;Oliver, Janet M.
背景：在人血液嗜碱性细胞中，高亲和力IgE受体Fc epsilon RI与多价抗原交联可激活导致炎症介质分泌和细胞因子产生的信号通路。嗜碱性粒细胞在哮喘发病机制中起重要作用，但对这些细胞产生的效应尚未有全面的研究。本文研究了嗜碱性细胞中一系列趋化因子和细胞因子的转录和释放。方法：笛卡儿抗体阵列是同时检测多种细胞因子和趋化因子的有效方法。结果经RT-PCR和酶联免疫吸附试验验证。这可以比较新鲜制备的外周血嗜碱性粒细胞对高亲和力IgE受体交联的反应，有和没有IL-3预孵育。结果：抗体阵列和RTPCR分析证实人血液嗜碱性细胞产生趋化因子MIP-5、eotaxin和GM-CSF。IL-3预孵育增强了哮喘和对照组嗜碱性细胞中IL-13、IL-8和编码MIP-5和GATA2的mRNA转录物的表达和释放。本文首次报道了瘦素mRNA在嗜碱性细胞中的转录、储存和释放。结论：对外周血嗜碱性粒细胞储存和释放的细胞因子和趋化因子的研究表明，哮喘患者和对照组患者即使在脱颗粒反应不同的情况下也具有相似的特征。证据表明，外周血嗜碱性粒细胞产生瘦素、GM-CSF、eotaxin和MIP-5。IL-3预孵育可促进IgE受体交联后IL-8的产生和释放。版权所有(c) 2007 S. Karger AG，巴塞尔。
Background: In human blood basophils, cross-linking the high-affinity IgE receptor Fc epsilon RI with multivalent antigen activates a signaling pathway leading to secretion of inflammatory mediators and cytokine production. Basophils are known to play an important role in the pathogenesis of asthma but there has been no comprehensive examination of the effectors these cells produce. Here a study of the transcription and release of a selection of chemokines and cytokines from basophils was undertaken. Methods: A Cartesian antibody array provided an effective method of assaying for multiple cytokines and chemokines simultaneously. Results were verified by RT-PCR and ELISA assays. This allowed the comparison of freshly prepared peripheral blood basophil responses to cross-linking of the high-affinity IgE receptor, with and without preincubation with IL-3. Results: Evidence that human blood basophils produce the chemokines MIP-5, eotaxin and GM-CSF was provided by antibody array and RTPCR analyses. Preincubation with IL-3 enhanced the expression and release of IL-13, IL-8 and mRNA transcripts encoding MIP-5 and GATA2 in basophils from both asthmatic and control subjects. Leptin mRNA transcription, storage and release in basophils are described for the first time. Conclusions: Surveying cytokine and chemokines stored and released by peripheral blood basophils shows that asthmatic and control subjects share similar profiles even when their degranulation responses are distinct. Evidence is provided for the production of leptin, GM-CSF, eotaxin and MIP-5 by peripheral blood basophils. IL-3 preincubation enhances the production and release of IL-8 upon IgE receptor cross-linking. Copyright (c) 2007 S. Karger AG, Basel.