Detection and characterization of bacterial nucleic acids in culture-negative synovial tissue and fluid samples from rheumatoid arthritis or osteoarthritis patients
Detection and characterization of bacterial nucleic acids in culture-negative synovial tissue and fluid samples from rheumatoid arthritis or osteoarthritis patients
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类风湿关节炎或骨关节炎患者培养阴性滑膜组织和体液样本中细菌核酸的检测和表征
DOI：
10.1038/s41598-018-32675-w
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发表时间：
2018-09-24
影响因子：
4.6
通讯作者：
Zhang, Lei
中科院分区：
文献类型：
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作者：
Zhao, Yan;Chen, Bin;Zhang, Lei
人类肠道微生物可以介导关节炎的发展-研究表明，某些细菌核酸可能存在于滑液(SF)中，并可能参与关节炎，尽管其潜在机制尚不清楚。用16S rRNA基因扩增序列测定了125例类风湿关节炎(RA)患者的15例滑膜组织(ST)和110例类风湿关节炎(RA)滑膜组织和58例骨关节炎(OA)患者的16例滑膜组织和42例SF标本中的细菌核酸群落。我们的结果显示，这些临床样本中存在丰富的细菌核酸多样性，包括所有183个样本中都存在卟啉单胞菌和类杆菌。类风湿关节炎(STRA)滑膜组织中以农杆菌属、丛毛单胞菌属、Kocuria属、Meithermus属和RhodoPlans较丰富。骨性关节炎(STEA)滑膜组织中以阿托波菌、嗜粘杆菌、粘红酵母、均匀类杆菌、罗氏菌、巨型杆菌、斑点杆菌、细毛杆菌、副流感嗜血杆菌、脆弱类杆菌、卟啉单胞菌、链球菌等较为丰富。类风湿关节炎(SFRA)的滑液中以致病韦氏杆菌、副流感嗜血杆菌、铜绿假单胞菌和淀粉密螺旋体较多，而骨性关节炎(SFOA)的滑液中以类杆菌较多。总体而言，这项研究证实了RA和OA皮损的SF和ST样本中细菌核酸的存在，并揭示了与疾病程度的潜在相关性。
Human intestinal microbes can mediate development of arthritis - Studies indicate that certain bacterial nucleic acids may exist in synovial fluid (SF) and could be involved in arthritis, although the underlying mechanism remains unclear. To characterize potential SF bacterial nucleic acids, we used 16S rRNA gene amplicon sequencing to assess bacterial nucleic acid communities in 15 synovial tissue (ST) and 110 SF samples from 125 patients with rheumatoid arthritis (RA) and 16 ST and 42 SF samples from 58 patients with osteoarthritis (OA). Our results showed an abundant diversity of bacterial nucleic acids in these clinical samples, including presence of Porphyromonas and Bacteroides in all 183 samples. Agrobacterium, Comamonas, Kocuria, Meiothermus, and Rhodoplanes were more abundant in synovial tissues of rheumatoid arthritis (STRA). Atopobium, Phascolarctobacterium, Rhodotorula mucilaginosa, Bacteroides uniformis, Rothia, Megasphaera, Turicibacter, Leptotrichia, Haemophilus parainfluenzae, Bacteroides fragilis, Porphyromonas, and Streptococcus were more abundant in synovial tissues of osteoarthritis (STOA). Veillonella dispar, Haemophilus parainfluenzae, Prevotella copri and Treponema amylovorum were more abundant in synovial fluid of rheumatoid arthritis (SFRA), while Bacteroides caccae was more abundant in the synovial fluid of osteoarthritis (SFOA). Overall, this study confirms existence of bacterial nucleic acids in SF and ST samples of RA and OA lesions and reveals potential correlations with degree of disease.