Differential miRNA Expression in Omental Adipose Tissue and in the Circulation of Obese Patients Identifies Novel Metabolic Biomarkers
Differential miRNA Expression in Omental Adipose Tissue and in the Circulation of Obese Patients Identifies Novel Metabolic Biomarkers
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DOI：
10.1210/jc.2010-2701
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发表时间：
2011-05-01
影响因子：
5.8
通讯作者：
Kerin, M. J.
中科院分区：
文献类型：
--
作者：
Heneghan, H. M.;Miller, N.;Kerin, M. J.
背景：燕麦油脂肪堆积与代谢综合征的发展有关，尽管其分子特征尚不清楚。MI(Cro)RNAs(MiRNAs)是一类小的非编码RNAs，在肥胖和代谢综合征中的作用尚不清楚，但已知其调节多种代谢过程。本研究旨在研究miRNA在大网膜、sc脂肪以及肥胖者和非肥胖者循环中的表达。方法：从50例肥胖症患者和50例非肥胖症患者的大网膜、sc脂肪组织和血液样本中提取miRNA。进行miRNA微阵列，并用RQ-PCR仪验证一组差异表达的miRNA。结果：大网膜和配对sc脂肪的miRNA表达谱是唯一的；这两个脂肪库之间的miRNA表达没有相关性。两种miRNAs(miR-17-5p和miR-132)在肥胖组和非肥胖组的表达差异有统计学意义(P=0.048和P=0.016)。这一表达模式反映在循环中，在肥胖受试者的血液中，这两个相同的miRNAs也显著失调。肥胖患者大网膜脂肪和血液中miRNA的表达与体重指数、空腹血糖和糖化血红蛋白显著相关。结论：肥胖患者脂肪组织和循环中的候选代谢miRNA发生了改变。燕麦油脂肪组织和全身miRNA水平反映了代谢综合征的组成部分，突出了它们作为这种复杂综合征的新生物标志物的潜力。(J临床内分泌Metab 96：E846-E850,2011)
Background: Omental fat accumulation is associated with development of the metabolic syndrome, although its molecular characteristics are poorly understood. Mi(cro)RNAs (miRNAs), a class of small noncoding RNAs, are known to regulate various metabolic processes, although their role in obesity and the metabolic syndrome is not clearly defined. This study sought to characterize the miRNA expression in omentum, sc fat and in the circulation of obese and nonobese individuals. Their potential as noninvasive metabolic biomarkers was also explored.Methods: miRNA was extracted from paired omentum and sc fat tissues, and blood samples, from a total of 50 obese and nonobese patients. A miRNA microarray was performed and a panel of differentially expressed miRNAs validated using RQ-PCR.Results: The miRNA expression profiles were unique for omentum and paired sc fat; no correlation in miRNA expression was observed between these two fat depots. Expression of two miRNAs (miR-17-5p and miR-132) differed significantly between obese and nonobese omental fat (P = 0.048 and P = 0.016). This expression pattern was reflected in the circulation in which these same two miRNAs were also significantly dysregulated in blood from obese subjects. The miRNA expression in omental fat and blood from obese patients correlated significantly with body mass index, fasting blood glucose, and glycosylated hemoglobin.Conclusion: This study demonstrates that candidate metabolic miRNAs are altered in adipose tissue and circulation of the obese. Omental fat tissue and systemic miRNA levels reflect components of the metabolic syndrome, highlighting their potential as novel biomarkers for this complex syndrome. (J Clin Endocrinol Metab 96: E846-E850, 2011)