Physiological Responses of Salinity-Stressed Vibrio sp and the Effect on the Biofilm Formation on a Nanofiltration Membrane
Physiological Responses of Salinity-Stressed Vibrio sp and the Effect on the Biofilm Formation on a Nanofiltration Membrane
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DOI：
10.1021/acs.est.6b02904
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发表时间：
2017-02-07
影响因子：
11.4
通讯作者：
Chong, Tzyy Haur
中科院分区：
文献类型：
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作者：
Kim, Lan Hee;Chong, Tzyy Haur
本研究评估了盐度对弧菌生理特性的影响。高回收率海水淡化过程中使用的纳滤 (NF) 膜片上的 B2 和生物膜形成。测试条件为0.6、1.2和2.4M氯化钠(NaCl)，分别相当于海水的盐度、水回收率50%和75%的盐水。高盐度抑制细胞生长速率，但增加活力和细菌膜完整性。此外，盐胁迫细菌的蛋白质和eDNA浓度在1.2和2.4M NaCl下增加。特别是，蛋白质浓度与 NaCl 浓度呈线性相关。类似地，盐度胁迫细菌在 NF 膜试样上（无渗透通量）上观察到较少的生物膜形成；然而，与对照相比，细胞外聚合物（EPS）的产生显着增加，并且蛋白质是生物膜形成的影响因素。这项研究表明，盐度胁迫的细菌很有可能在膜表面造成生物污垢，因为细菌仍然保持细胞活性并过量产生 EPS。盐度胁迫细菌形成生物膜的潜力尚未见报道。因此，这些发现对于理解高盐度环境中膜生物污染的机制具有重要意义。
This study evaluated the effects of salinity on the physiological characteristics of Vibrio sp. B2 and biofilm formation on nanofiltration (NF) membrane coupons used in the high recovery seawater desalination process. The test conditions were at 0.6, 1.2, and 2.4 M sodium chloride (NaCI), equivalent to salinity of seawater, brine at 50% and 75% water recovery, respectively. High salinity inhibited the cell growth rate but increased the viability and bacterial membrane integrity. In addition, protein and eDNA concentrations of salinity-stressed bacteria were increased at 1.2 and 2.4 M NaCI. In particular, protein concentration was linearly correlated with the NaC1 concentration. Similarly, less biofilm formation on the NF membrane coupon (without permeation flux) was observed by the salinity stressed bacteria; however, the production of extracellular polymeric substances (EPS) was significantly increased as compared to control, and protein was an influential factor for biofilm formation. This study shows that salinity-stressed bacteria have a high potential to cause biofouling on membrane surface as the bacteria still maintain the cell activity and overproduce EPS. The potential of biofilm formation by the salinity-stressed bacteria has not been reported. Therefore, the findings are important to understand the mechanisms of membrane biofouling in a high salinity environment.