CHARACTERIZATION OF MACROPHAGE-LIKE CELLS IN THE EXTERNAL LAYERS OF HUMAN SMALL AND LARGE-INTESTINE
CHARACTERIZATION OF MACROPHAGE-LIKE CELLS IN THE EXTERNAL LAYERS OF HUMAN SMALL AND LARGE-INTESTINE
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DOI：
10.1007/bf00328013
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发表时间：
1992-11-01
影响因子：
3.6
通讯作者：
RUMESSEN, JJ
中科院分区：
文献类型：
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作者：
MIKKELSEN, HB;RUMESSEN, JJ
用免疫组织化学、组织化学和电子显微镜等方法对人小肠和大肠外层的巨噬细胞样细胞进行了观察。利用免疫组织化学和一些单抗，评估了巨噬细胞表型亚群的存在和分布。巨噬细胞样细胞均以识别CD68抗原的抗体EBM11、识别CD11b的C3bi和识别蛋白150、95(CD11c)的抗体部分识别。外肌层的巨噬细胞样细胞表达MHC-II类抗原，而浆膜下层和粘膜下层的巨噬细胞样细胞呈HLA-DR阳性。外肌层中的巨噬细胞样细胞多为酸性磷酸酶阴性，在电子显微镜水平上发现它们具有巨噬细胞的特征：初级溶酶体、包被的小泡和小凹。然而，次级溶酶体很少存在。未见Birbeck颗粒。结果表明，在人的小肠和大肠的外肌层中，存在大量特殊类型的巨噬细胞。讨论了这种细胞类型是否在胃肠运动中起作用和/或具有免疫功能。
In the external layers of human small and large intestine macrophage-like cells were characterized by immunohistochemical, histochemical and electron-microscopical methods. Using immunohistochemistry and a number of monoclonal antibodies, the presence and distribution of phenotypic subpopulations of macrophages were evaluated. In all locations macrophage-like cells were identified with antibody EBM11, which recognizes CD68 antigen, C3bi which recognizes CD11b, and partly with an antibody which recognizes protein 150,95 (CD11c). Macrophage-like cells in the external muscle layer were HLA-DR-positive (expressing the MHC class-II antigen), in contrast to macrophage-like cells in the subserosa and submucosa. Macrophage-like cells in the external muscle layer were mostly acid phosphatase-negative, and at the electron-microscopic level they were found to have features of macrophages: primary lysosomes, coated vesicles and pits. However, very few secondary lysosomes were present. Birbeck granules were not observed. It is concluded that in the external muscle layer of human small and large intestine numerous macrophages of a special type are present. It is discussed whether this cell type plays a role in gastrointestinal motility and/or has an immunological function.