Overexpression of HVA1 gene from barley generates tolerance to salinity and water stress in transgenic mulberry (Morus indica)
Overexpression of HVA1 gene from barley generates tolerance to salinity and water stress in transgenic mulberry (Morus indica)
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DOI：
10.1007/s11248-007-9145-4
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发表时间：
2008-08-01
影响因子：
3
通讯作者：
Khurana, Paramjit
中科院分区：
文献类型：
--
作者：
Lal, Shalini;Gulyani, Vibha;Khurana, Paramjit
晚期胚胎发生丰富（莱亚）蛋白是主要在植物中发现的一大组亲水性蛋白的成员。大麦hva1基因编码第3组莱亚蛋白，并由阿坝和水分亏缺条件诱导。我们在这里报告的组成型启动子下，通过农杆菌介导的转化桑树hva1过表达。对转基因植株的分子生物学分析表明，转基因在转化子中稳定整合和表达。将转基因植物置于模拟盐和干旱胁迫条件下，研究hva 1在赋予植物耐受性中的作用。转基因植株在盐胁迫和干旱胁迫下均表现出较好的细胞膜稳定性（CMS）、光合产量、较低的光氧化损伤和较好的水分利用效率。在盐胁迫下，转基因植物的脯氨酸浓度比非转基因植物增加许多倍，并且在水分亏缺条件下，脯氨酸仅在非转基因植物中积累。结果还表明，HVA1蛋白的产生有助于通过保护质膜的膜稳定性以及叶绿体膜免受非生物胁迫下的损伤来改善转基因桑葚的性能。有趣的是，观察到hva 1赋予转基因植物对各种胁迫条件不同程度的耐受性。在分析胁迫耐受性的品系中，与非转基因植物相比，转基因品系ST 8相对更耐盐，ST 30、ST 31更耐干旱，并且品系ST 11和ST 6在盐胁迫和干旱胁迫条件下反应良好。因此，hva 1似乎赋予桑葚在非生物胁迫下的广谱耐受性。
Late embryogenesis abundant (LEA) proteins are members of a large group of hydrophilic proteins found primarily in plants. The barley hva1 gene encodes a group 3 LEA protein and is induced by ABA and water deficit conditions. We report here the over expression of hva1 in mulberry under a constitutive promoter via Agrobacterium-mediated transformation. Molecular analysis of the transgenic plants revealed the stable integration and expression of the transgene in the transformants. Transgenic plants were subjected to simulated salinity and drought stress conditions to study the role of hva1 in conferring tolerance. The transgenic plants showed better cellular membrane stability (CMS), photosynthetic yield, less photo-oxidative damage and better water use efficiency as compared to the non-transgenic plants under both salinity and drought stress. Under salinity stress, transgenic plants show many fold increase in proline concentration than the non-transgenic plants and under water deficit conditions proline is accumulated only in the non-transgenic plants. Results also indicate that the production of HVA1 proteins helps in better performance of transgenic mulberry by protecting membrane stability of plasma membrane as well as chloroplastic membranes from injury under abiotic stress. Interestingly, it was observed that hva1 conferred different degrees of tolerance to the transgenic plants towards various stress conditions. Amongst the lines analysed for stress tolerance transgenic line ST8 was relatively more salt tolerant, ST30, ST31 more drought tolerant, and lines ST11 and ST6 responded well under both salinity and drought stress conditions as compared to the non-transgenic plants. Thus hva1 appears to confer a broad spectrum of tolerance under abiotic stress in mulberry.