Magnetic properties of Acidithiobacillus ferrooxidans.
Magnetic properties of Acidithiobacillus ferrooxidans.
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DOI：
10.1016/j.msec.2013.05.046
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发表时间：
2013-10
期刊：
影响因子：
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通讯作者：
Lei Yan;Shuang Zhang;Peng Chen;Weidong Wang;Yan-jie Wang;Hongyu Li
中科院分区：
文献类型：
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作者：
Lei Yan;Shuang Zhang;Peng Chen;Weidong Wang;Yan-jie Wang;Hongyu Li
了解趋磁细菌（MTB）的磁性在生命科学、地球科学、生物矿化、生物磁学和行星科学等领域具有重要意义。嗜酸氧化亚铁硫杆菌（Acidithiobacillus ferrooxidans，At.铁氧化物（ferrooxidans），通过氧化亚铁和各种还原的无机硫化合物获得能量，可以合成细胞内的磁铁矿磁小体。然而，这种微生物的磁性仍然未知。本文采用透射电子显微镜（TEM）、扫描电子显微镜（SEM）、X射线衍射（XRD）、振动样品磁强计（VSM）、磁热重分析（MTGA）和低温磁强计等手段对At的磁性进行了全面的研究。氧化亚铁结果表明，每个细胞仅含1 ~ 3个磁铁矿磁小体，排列不规则。结果表明，磁小体基本上处于稳定的单畴（SD）状态，但也发现了超顺磁性（SP）磁铁矿颗粒，煅烧细菌表现出铁磁性，居里温度为454 °C，磁化率为16.36 mT。δFC/δZFC= 1.27，表明At中不存在完整的磁小体链。氧化亚铁我们的研究结果为细菌磁小体的生物矿化和At的磁性提供了新的认识。氧化亚铁
Understanding the magnetic properties of magnetotactic bacteria (MTBs) is of great interest in fields of life sciences, geosciences, biomineralization, biomagnetism, and planetary sciences. Acidithiobacillus ferrooxidans (At. ferrooxidans), obtaining energy through the oxidation of ferrous iron and various reduced inorganic sulfur compounds, can synthesize intracellular magnetite magnetosomes. However, the magnetic properties of such microorganism remain unknown. Here we used transmission electronmicroscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD) assay, vibrating sample magnetometer (VSM), magneto–thermogravimetric analysis (MTGA), and low temperature magnetometry to comprehensively investigate the magnetic characteristics ofAt. ferrooxidans. Results revealed that each cell contained only 1 to 3 magnetite magnetosomes, which were arranged irregularly. The magnetosomes were generally in a stable single-domain (SD) state, but superparamagnetic (SP) magnetite particles were also found. The calcined bacteria exhibited a ferromagnetic behavior with a Curie Temperature of 454 °C and a coercivity of 16.36 mT. Additionally, the low delta ratio (δFC/δZFC= 1.27) indicated that there were no intact magnetosome chains inAt. ferrooxidans. Our results provided the new insights on the biomineralization of bacterial magnetosomes and magnetic properties ofAt. ferrooxidans.