A comparative study of the characterization of miR-155 in knockout mice.
A comparative study of the characterization of miR-155 in knockout mice.
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DOI：
10.1371/journal.pone.0173487
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Tang Y
中科院分区：
文献类型：
--
作者：
Zhang D;Cui Y;Li B;Luo X;Li B;Tang Y
MiR-155是最重要的miRNAs之一，在许多生物学过程中发挥着非常重要的作用。然而，很少有研究在正常生理条件下在小鼠身上表征这种miRNA。我们的目的是通过比较分析来确定miR-155在体内的特征。在我们的研究中，我们比较了miR-155基因敲除(KO)小鼠和C57BL/6野生型(WT)小鼠，以便在正常生理条件下对miR-155小鼠进行特征描述，包括繁殖性能分析、生长曲线、超声评估、血液学检查和组织病理学分析。这些分析表明，两组之间的主要评价指标没有显著差异。所有小鼠的生长和发育几乎正常，对照组和模型组之间没有差异。通过比较分析和总结近年来发表的相关研究，我们发现miR-155对于8周龄小鼠的正常生理过程并不是必需的。在出生后42天内，MIR-155缺乏对自然衰老小鼠的发育和生长没有影响。因此，研究miR-155的复杂生物学功能需要进一步使用KO小鼠模型。
miR-155 is one of the most important miRNAs and plays a very important role in numerous biological processes. However, few studies have characterized this miRNA in mice under normal physiological conditions. We aimed to characterize miR-155 in vivo by using a comparative analysis. In our study, we compared miR-155 knockout (KO) mice with C57BL/6 wild type (WT) mice in order to characterize miR-155 in mice under normal physiological conditions using many evaluation methods, including a reproductive performance analysis, growth curve, ultrasonic estimation, haematological examination, and histopathological analysis. These analyses showed no significant differences between groups in the main evaluation indices. The growth and development were nearly normal for all mice and did not differ between the control and model groups. Using a comparative analysis and a summary of related studies published in recent years, we found that miR-155 was not essential for normal physiological processes in 8-week-old mice. miR-155 deficiency did not affect the development and growth of naturally ageing mice during the 42 days after birth. Thus, studying the complex biological functions of miR-155 requires the further use of KO mouse models.