Long-term growth and differentiation of Xenopus oocytes in a defined medium.
Long-term growth and differentiation of Xenopus oocytes in a defined medium.
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非洲爪蟾卵母细胞在特定培养基中的长期生长和分化。
DOI：
10.1073/pnas.75.11.5534
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发表时间：
1978
影响因子：
11.1
通讯作者：
Z. Misulovin
中科院分区：
文献类型：
--
作者：
R. Wallace;Z. Misulovin
从卵泡中分离出大小在0.15- 0.78 mm3之间的非洲爪蟾卵母细胞，在指定的培养基中培养28天。卵母细胞的平均生长速率为0.021 mm3。在没有胰岛素和0.030 mm3的情况下，第1天。在胰岛素存在的第1天。后者的平均生长速率对应于迄今为止报道的卵母细胞在体内的最快生长速率。体外培养的卵母细胞可以达到至少1.43 mm3的大小，这比通常在体内发现的最大尺寸要大。在体外生长期间；卵母细胞也获得正常的色素模式，一旦达到约0.7 mm3，作为对外用黄体酮的反应，卵母细胞有能力经历完全成熟。这些结果表明，非洲爪蟾卵母细胞能够在体外生长和分化。
Xenpus laevis oocytes over a size range of 0.15--0.78 mm3 were dissected from their follicles and cultured in a defined medium for up to 28 days. Oocytes grew at average rates of 0.021 mm3.day-1 in the absence of insulin and 0.030 mm3.day-1 in the presence of insulin. The latter average growth rate corresponds to the fastest growth rate reported to date for oocytes in vivo. Oocytes grown in vitro can reach a size of at least 1.43 mm3, which is larger than the maximum size generally found in vivo. During growth in vitro; oocytes also acquire both a normal pigment pattern and, once they reach about 0.7 mm3, the ability to undergo complete maturation as a response to externally applied progesterone. These results show that Xenopus oocytes freed of their follicular investments are able to grow and differentiate in vitro.