Birth and life of tissue macrophages and their migration in embryogenesis and inflammation in medaka
Birth and life of tissue macrophages and their migration in embryogenesis and inflammation in medaka
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DOI：
10.1189/jlb.0806526
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发表时间：
2007-01-01
影响因子：
5.5
通讯作者：
Wittbrodt, Joachim
中科院分区：
文献类型：
--
作者：
Grabher, Clemens;Cliffe, Adam;Wittbrodt, Joachim
巨噬细胞检测并向损伤和感染部位迁移是免疫反应的第一步。在这里，我们直接拍摄了转基因青冈鱼体内巨噬细胞的生成和迁移。巨噬细胞生下来就是经常分裂的、不活动的细胞，具有球形的形态，分化成扁平的、高度活动的细胞。它们保持有丝分裂活动，同时扩散到整个身体。细胞不仅在定向迁移中遵循受限的路径，而且在巡逻时也是如此。沿着这些路径，巨噬细胞迅速巡逻组织，并对远距离的创伤和细菌感染做出反应。一旦受伤，它们的速度和迁徙的持久性就会增加。具体地说，靶向PI3-激酶亚型可以有效地阻断损伤反应，并导致明显抑制细胞的运动和趋化作用。我们的研究为未成熟和迁徙的巨噬细胞的特性提供了原位的见解，并提出了一个独特的模型来进一步测试体内的调节化合物。
Macropbages detecting and migrating toward sites of injury and infection represent one of the first steps in an immune response. Here we directly image macrophage birth and migration in vivo in transgenic medaka fish. Macrophages are born as frequently dividing, immotile cells with spherical morphology that differentiate into flat, highly motile cells. They retain mitotic activity while spreading over the entire body. Cells follow restricted paths not only in directed migration, but also during patrolling. Along those paths the macrophages rapidly patrol the tissue and respond to wounding and bacterial infection from long distances. Upon injury they increase their speed and migratory persistence. Specifically targeting PI3-kinase isoforms efficiently blocks the wounding response and results in a distinct inhibition of cell motility and chemotaxis. Our study provides in situ insights into the properties of immature and migratory macrophages and presents a unique model to further test modulating compounds in vivo.