Excluded volume effect within the continuous model for the fluorescence energy transfer.
Excluded volume effect within the continuous model for the fluorescence energy transfer.
复制标题
排除荧光能量转移连续模型中的体积效应。
DOI：
10.1016/s0006-3495(02)75291-0
复制
发表时间：
2002
影响因子：
3.4
通讯作者：
Klushin,Leonid
中科院分区：
文献类型：
--
作者：
Tcherkasskaya,Olga;Gronenborn,AngelaM;Klushin,Leonid
我们考虑并讨论了电子能量在无限空间中连续分布的供体分子和受体分子之间的转移。特别地，考虑到排除体积，计算供体的整体平均荧光强度衰减。后者可能与有限的分子大小或任何其他空间限制有关，这些限制是由超结构对荧光团分布施加的。结果表明，在使用排除体积的系统中，施主衰变的时间依赖性比由简化的拉伸指数模型预测的更复杂。我们确定了两个不同的时间制度之间的交叉细化衰减，并证明其与两个竞争参数的相关性：rm，其特征相互作用的分子之间的最小距离，andR0，这是有关的分子相互作用的强度。在这种情况下，从拉伸指数模型恢复的能量转移的“表观维数”忽略了交叉，并且可能是相当误导的。为此提供了基本的理论考虑。
We consider and discuss the transfer of electronic energy between donor and acceptor molecules, both continuously distributed in an infinite space. In particular, the ensemble-average fluorescence intensity decay for the donor was calculated, taking into account the excluded volume. The latter may be associated either with finite molecular size or any other spatial restrictions, which are imposed on fluorophore distribution by a superstructure. Results show that in a system using excluded volume, the time dependence in donor decay is more complex compared to that predicted by a simplified stretched exponential model. We identify a crossover between two distinct time regimes in the refined decay and demonstrate its correlation with two competing parameters:rm, which characterizes the minimal distance between interacting molecules, andR0, which is related to the strength of the molecular interactions. In this context, the "apparent dimensionality" of the energy transfer recovered from the stretched exponential model ignores the crossover, and may be quite misleading. Basic theoretical considerations to that end are provided.