Crystallization, X-ray diffraction analysis and SIRAS/molecular-replacenent phasing of three crystal forms of Anabaena sensory rhodopsin transducer.
Crystallization, X-ray diffraction analysis and SIRAS/molecular-replacenent phasing of three crystal forms of Anabaena sensory rhodopsin transducer.
复制标题
鱼腥藻感觉视紫红质传感器三种晶型的结晶、X 射线衍射分析和 SIRAS/分子替代定相。
DOI：
10.1107/s1744309106008359
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Luecke,Hartmut
中科院分区：
文献类型：
--
作者：
Vogeley,Lutz;Luecke,Hartmut
Anabaena sensory rhodopsin transducer (ASRT) is a 14.7 kDa soluble signaling protein associated with the membrane-embedded light receptor Anabaena sensory rhodopsin (ASR) from Anabaena sp., a freshwater cyanobacterium. Crystals of ASRT were obtained in three different space groups, P4, C2 and P212121, which diffract to 1.8, 2.1 and 2.0 Å, respectively. Phases for one of these crystal forms (P4) were obtained by SIRAS phasing using an iodide quick-soak derivative and a partial model was built. Phases for the remaining crystal forms were obtained by molecular replacement using the partial model from the P4 crystal form.
