Characterization of fungal microbiota on normal ocular surface of humans
Characterization of fungal microbiota on normal ocular surface of humans
复制标题
人类正常眼表面真菌微生物群的特征
DOI：
10.1016/j.cmi.2019.05.011
复制
发表时间：
2020
影响因子：
14.2
通讯作者：
Huang Y.
中科院分区：
文献类型：
--
作者：
Wang Y.;Chen H.;Xia T.;Huang Y.
ObjectivesOur objective was to characterize the fungal microbiota on normal ocular surface of humans with the culture based method and high-throughput sequencing approach.MethodsA total of 45 adults were recruited from a urban community，and 90 conjunctival swabs were obtained，one from each eye of each participant.从每个参与者的两个拭子之一被随机选择，并分配到内部转录间隔区（ITS）测序，和其他进行常规的真菌culture.Results4丝状真菌分离出45个样本使用的培养为基础的方法，桔青霉，尼日尔曲霉，PhialophoraandTrichoderma。另外45份样本中，18份样本PCR扩增阳性，送去后续ITS测序。共生成518703个有效读段，并分配到467个操作分类单元。共鉴定出4门94属。两门，担子菌门（78.67%）和子囊菌门（19.54%）;马拉色菌属（Malassezia（74.65%），红酵母属（1.93%），戴维氏菌属（1.89%），曲霉属（1.25%）和链格孢属（0.61%），其占真菌微生物组的>80%并且在>80%的测试个体中存在，构成了正常眼表上可能的“核心真菌类群”。结论使用高通量测序方法鉴定了人类正常眼表上的真菌微生物组，为进一步研究真菌微生物群在眼部健康和疾病中的潜在作用提供了基础。
ObjectivesOur objective was to characterize the fungal microbiota on normal ocular surface of humans with the culture-based method and high-throughput sequencing approach.MethodsA total of 45 adults were recruited from an urban community, and 90 conjunctival swabs were obtained, one from each eye of each participant. One of the two swabs from each participant was randomly chosen and allocated to internal transcribed spacer (ITS) sequencing, and the other was subjected to conventional fungal cultivation.ResultsFour filamentous fungi were isolated from the 45 samples using the culture-based method,Penicillium citrinum,Aspergillus niger,PhialophoraandTrichoderma. In the other 45 samples, 18 samples were positive for PCR amplification and sent for subsequent ITS sequencing. A total of 518 703 valid reads were generated and assigned into 467 operational taxonomic units. Overall, 4 phyla and 94 genera were identified. Two phyla,Basidiomycota(78.67%) andAscomycota(19.54%), and five genera,Malassezia(74.65%),Rhodotorula(1.93%),Davidiella(1.89%),Aspergillus(1.25%) andAlternaria(0.61%), which accounted for >80% of the fungal microbiome and presented in >80% of the individuals tested, constituted the possible ‘core fungal taxa’ on normal ocular surface.ConclusionsThe fungal microbiome on normal ocular surface of humans was identified using the high-throughput sequencing method, providing a basis for further investigations on the potential role of the fungal microbiota in ocular health and disease.