Increased recruitment of TATA-binding protein to the promoter by transcriptional activation domains in vivo.
Increased recruitment of TATA-binding protein to the promoter by transcriptional activation domains in vivo.
复制标题
通过体内转录激活域增加 TATA 结合蛋白向启动子的募集。
DOI：
10.1126/science.7939664
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Struhl,K
中科院分区：
文献类型：
--
作者：
Klein,C;Struhl,K
The rate at which the TATA-binding protein (TBP) interacts with the TATA element and promotes transcription by RNA polymerase II was determined in yeast cells. A TBP derivative with altered TATA-element specificity was rapidly induced, and transcription from promoters with appropriately mutated TATA elements was measured. Without a functional activator protein, basal transcription was observed only after a lag of several hours. In contrast, GCN4-activated transcription occurred rapidly upon induction of the TBP derivative. These results suggest that accessibility of TBP to the chromatin template in vivo is rate limiting and that activation domains increase recruitment of TBP to the promoter.
