Comparison of IL-17 production by helper T cells among atopic and nonatopic asthmatics and control subjects
Comparison of IL-17 production by helper T cells among atopic and nonatopic asthmatics and control subjects
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DOI：
10.1159/000085432
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发表时间：
2005-01-01
影响因子：
2.8
通讯作者：
Mori, A
中科院分区：
文献类型：
--
作者：
Hashimoto, T;Akiyama, K;Mori, A
背景：T细胞、嗜酸性粒细胞和中性粒细胞在支气管哮喘的发病机制中起重要作用。哮喘中影响中性粒细胞聚集和活化的机制仍然相对模糊。有数据表明，IL-17由T细胞产生，并导致气道上皮细胞释放嗜中性粒细胞动员细胞因子，并以这种方式调节气道嗜中性粒细胞。研究方法：用固定化抗CD 3抗体（Ab）加可溶性抗CD 28抗体或粉尘螨（Df）提取物刺激特应性哮喘（AA）、非特应性哮喘（NA）和正常对照（NC）的外周血单个核细胞（PBMC）。从AA的PBMC中建立DF反应性T细胞克隆，并在各种刺激剂的存在下培养。通过特异性ELISA测定所得上清液的IL-2、IL-4、IL-5、IL-13、IL-17和IFN-γ。结果：AA、NA和NC的PBMC在固定化抗CD 3抗体和可溶性抗CD 28抗体刺激下均产生IL-17。响应于Df提取物的IL-17产生仅在AA中显著诱导。用固定化抗CD 3 Ab加可溶性抗CD 28 Ab刺激的T细胞克隆产生的IL-17的量与IL-4的量呈负相关，但仅弱相关，但与IL-2、IL-5、IL-13和IFN-γ的产生不相关。结论：致敏AA患者外周血中存在对Df抗原产生IL-17的T细胞。IL-17的产生可能受到不同于Th 1与Th 2分化的独特机制的调节。版权所有（C）2005 S. Karger AG，巴塞尔。
Background: T cells, eosinophils, and neutrophils are strongly involved in the pathogenesis of bronchial asthma. Mechanisms that influence neutrophil accumulation and activation in asthma still remain relatively obscure. There is data indicating that IL-17 is produced by T cells and causes the release of neutrophil-mobilizing cytokines from airway epithelial cells, and that in this way it may regulate airway neutrophilia. Methods: Peripheral blood mononuclear cells (PBMC) obtained from atopic asthmatics (AA), nonatopic asthmatics (NA), and normal control subjects (NC) were stimulated by immobilized anti-CD3 antibody (Ab) plus soluble anti-CD28 Ab or Dermatophagoides farinae (Df) extract. Df-reactive T cell clones were established from PBMC of AA and cultured in the presence of various stimulants. The resulting supernatants were assayed for IL-2, IL-4, IL-5, IL-13, IL-17, and IFN-gamma by specific ELISAs. Results: PBMC obtained from AA, NA, and NC all produced IL-17 upon immobilized anti-CD3 Ab plus soluble anti-CD28 Ab stimulation. IL-17 production in response to Df extract was significantly induced only in AA. The amount of IL-17 produced by T cell clones stimulated with immobilized anti-CD3 Ab plus soluble anti-CD28 Ab was negatively, but only weakly, correlated with that of IL-4, but not correlated with IL-2, IL-5, IL-13, and IFN-gamma production. Conclusion: T-cells producing IL-17 in response to Df antigen exist in the peripheral blood of the sensitized AA. IL-17 production might be regulated by unique mechanisms different from those governing Th1 versus Th2 differentiation. Copyright (C) 2005 S. Karger AG, Basel.