Water-soluble chromenylium dyes for shortwave infrared imaging in mice.
Water-soluble chromenylium dyes for shortwave infrared imaging in mice.
复制标题
DOI：
10.1016/j.chempr.2023.08.021
复制
发表时间：
2023-09
期刊：
影响因子：
23.5
通讯作者：
Shang Jia;Eric Y Lin;Emily B. Mobley;Irene Lim;Lei Guo;Shivakrishna Kallepu;Philip S. Low;Ellen M. Sletten
中科院分区：
文献类型：
--
作者：
Shang Jia;Eric Y Lin;Emily B. Mobley;Irene Lim;Lei Guo;Shivakrishna Kallepu;Philip S. Low;Ellen M. Sletten
与使用可见光和近红外波长相比，使用短波红外（SWIR）光（1，000 - 2，000 nm）的体内成像受益于更深的穿透力和更高的分辨率。然而，生物相容性SWIR造影剂的开发仍然具有挑战性。尽管最近的进展，小分子SWIR荧光团往往受到其显着的疏水性。我们报告了一个平台，用于生成一个面板的可溶性和功能性染料的SWIR成像的后期功能化的通用荧光团中间体，提供水溶性染料与明亮的SWIR荧光血清。具体而言，四磺酸盐衍生物仅用0.05 nmol染料就能实现脉管系统的清晰视频速率成像，而四铵染料显示出用于跟踪肿瘤生长的强细胞保留。此外，膦酸酯官能团的掺入使得能够对清醒小鼠中的骨进行成像。这种模块化设计提供了对现有SWIR荧光团的简易衍生化的见解，以将溶解度和生物活性引入活体生物成像。
In vivoimaging using shortwave infrared (SWIR) light (1,000–2,000 nm) benefits from deeper penetration and higher resolution compared with using visible and near-infrared wavelengths. However, the development of biocompatible SWIR contrast agents remains challenging. Despite recent advancements, small-molecule SWIR fluorophores are often hindered by their significant hydrophobicity. We report a platform for generating a panel of soluble and functional dyes for SWIR imaging by late-stage functionalization of a versatile fluorophore intermediate, affording water-soluble dyes with bright SWIR fluorescence in serum. Specifically, a tetra-sulfonate derivative enables clear video-rate imaging of vasculature with only 0.05 nmol dye, and a tetra-ammonium dye shows strong cellular retention for tracking of tumor growth. Additionally, incorporation of phosphonate functionality enables imaging of bones in awake mice. This modular design provides insights into facile derivatization of existing SWIR fluorophores to introduce both solubility and bioactivity towardin vivobioimaging.