A new murine CD4+ T cell subset with an unrestricted cytokine profile.
A new murine CD4+ T cell subset with an unrestricted cytokine profile.
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DOI：
10.4049/jimmunol.143.2.518
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发表时间：
1989-07
影响因子：
4.4
通讯作者：
G. Firestein;W. Roeder;J. A. Laxer;K. Townsend;C. Weaver;J. Hom;J. Linton;B. Torbett;A. Glasebrook
中科院分区：
文献类型：
--
作者：
G. Firestein;W. Roeder;J. A. Laxer;K. Townsend;C. Weaver;J. Hom;J. Linton;B. Torbett;A. Glasebrook
CD4+ T 细胞克隆源自免疫匙孔血蓝蛋白的小鼠，以表征新分离克隆的细胞因子谱。令人惊讶的是，一些克隆具有不受限制的特征，在 Con A 或 Ag 刺激后产生 IL-2、IL-3、IL-4、IFN-γ 和 TNF。 IL-2 和 IL-4 的共同产生在 mRNA 水平上得到证实。衍生出含有 IL-2 和 IL-4 的 RNA 转录物并分泌的亚克隆，从而证实了原始 T 细胞克隆的克隆性。在 Con A 刺激下表达不受限制的细胞因子谱的 CD4+ T 细胞克隆也从免疫其他 Ag（鸡蛋溶菌酶、OVA 或 II 型胶原蛋白）的小鼠中分离出来。这些数据表明，从免疫小鼠中新分离出的 CD4+ T 细胞克隆不一定分为 Th1 和 Th2 亚群。我们建议将这种具有不受限制的细胞因子产生模式的新鼠 CD4+ 细胞亚群称为 Th0。
CD4+ T cell clones were derived from mice immunized to keyhole limpet hemocyanin to characterize the cytokine profiles of newly isolated clones. Surprisingly, several of the clones had an unrestricted profile, producing IL-2, IL-3, IL-4, IFN-gamma, and TNF after either Con A or Ag stimulation. The coproduction of IL-2 and IL-4 was confirmed at the mRNA level. Subclones were derived which contained RNA transcripts for, as well as secreted, both IL-2 and IL-4 thus confirming the clonality of the original T cell clones. CD4+ T cell clones that expressed an unrestricted cytokine profile upon Con A stimulation were also isolated from mice immunized to other Ag (hen egg lysozyme, OVA, or type II collagen). These data indicate that CD4+ T cell clones newly isolated from immunized mice do not necessarily segregate into the Th1 and Th2 subsets. We propose this new murine CD4+ cell subset with an unrestricted pattern of cytokine production be called Th0.