Diacylglycerol stimulates DNA synthesis and cell division in mouse 3T3 cells: role of Ca2+-sensitive phospholipid-dependent protein kinase.
Diacylglycerol stimulates DNA synthesis and cell division in mouse 3T3 cells: role of Ca2+-sensitive phospholipid-dependent protein kinase.
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二酰基甘油刺激小鼠 3T3 细胞中的 DNA 合成和细胞分裂：Ca2 敏感性磷脂依赖性蛋白激酶的作用。
DOI：
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发表时间：
1984
影响因子：
11.1
通讯作者：
J. Sinnett
中科院分区：
文献类型：
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作者：
E. Rozengurt;A. Rodríguez;M. Coombs;J. Sinnett
在瑞士3T3细胞的单层培养中，合成的二酰基甘油1-油基-2-乙酰甘油直接与[3H]phorbol 12,13-二丁酸酯竞争共同结合位点，并迅速刺激Mr 80,000细胞蛋白的磷酸化，该蛋白最近被证明反映了完整细胞中蛋白激酶C的激活。因此，该二酰基甘油提供了一个有用的工具，以确定外源添加的二酰基甘油是否可以模拟有效的肿瘤启动子佛波酯，在静止细胞中诱导DNA合成和细胞分裂。我们发现OAG与胰岛素和其他生长因子协同作用，刺激细胞增殖的重新启动，并且几条证据表明，OAG通过刺激完整3T3细胞中的蛋白激酶C活性，与phorbol酯共享有丝分裂作用的共同途径。这些发现支持了二酰基甘油代表内源性佛波酯类似物的假设，并提出了在质膜中产生的二酰基甘油可能作为静止细胞的有丝分裂信号的可能性。
The synthetic diacylglycerol 1-oleoyl-2-acetylglycerol competes directly with [3H]phorbol 12,13-dibutyrate for common binding sites in monolayer cultures of Swiss 3T3 cells and rapidly stimulates the phosphorylation of a Mr 80,000 cellular protein that has recently been shown to reflect the activation of protein kinase C in intact cells. Thus, this diacylglycerol provided a useful tool to determine whether exogenously added diacylglycerols can mimic the potent tumor promoter phorbol ester in eliciting DNA synthesis and cell division in quiescent cells. We found that OAG acts synergistically with insulin and other growth factors to stimulate reinitiation of cell proliferation, and several lines of evidence indicate that OAG shares with phorbol esters a common pathway of mitogenic action via stimulation of protein kinase C activity in intact 3T3 cells. The findings support the hypothesis that diacylglycerols represent endogenous analogs of phorbol esters and raise the possibility that diacylglycerols generated in the plasma membrane could act as a mitogenic signal for quiescent cells.