A novel recombinant of Hepatitis B virus genotypes G and C isolated from a Thai patient with hepatocellular carcinoma
A novel recombinant of Hepatitis B virus genotypes G and C isolated from a Thai patient with hepatocellular carcinoma
复制标题
DOI：
10.1099/vir.0.81241-0
复制
发表时间：
2005-11-01
影响因子：
3.8
通讯作者：
Poovorawan, Y
中科院分区：
文献类型：
--
作者：
Suwannakarn, K;Tangkijvanich, P;Poovorawan, Y
不同基因型乙型肝炎病毒（HBV）之间发生基因组重组从而产生杂交株的情况已日益增多有文献记载。在本研究中，报道了从一名泰国肝细胞癌患者体内分离出的一种新型的HBV基因型G和C的重组体。基于对S、P和X基因以及全基因组的系统发育分析，该HBV分离株在基因型G内的一个分支上聚类，但在对C基因进行分析时与基因型C聚类。利用SIMPLOT程序和靴带扫描分析，重组断点位于前C区/核心区的第1860和2460位核苷酸处。在该分离株中未发现原始基因型G的特征，包括核心区36bp的插入以及前C区的双终止密码子。这些数据应鼓励进一步研究参与与其他基因型重组的HBV基因型G的流行病学和病毒学特征。
Genomic recombination between different genotypes of Hepatitis B virus (HBV) resulting in hybrid strains has been increasingly documented. In this study, a novel recombinant of HBV genotypes G and C isolated from a Thai patient with hepatocellular carcinoma is reported. Based on phylogenetic analyses of the S, P and X genes and the entire genome, the HBV isolate clustered on a branch within genotype G, but clustered with genotype C on analysis of the C gene. Using the program SIMPLOT and bootscanning analysis, the recombination breakpoints were located at nt 1860 and 2460 of the precore/core region. The hallmarks of the original genotype G, including a 36 bp insertion in the core region and dual stop codons in the precore region, were not identified in this isolate. These data should encourage further investigations on the epidemiological and virological characteristics of HBV genotype G involved in recombination with other genotypes.