Reassessment of the role of splenic leukocyte oxidative activity and macrophage activation in expression of immunity to malaria.
Reassessment of the role of splenic leukocyte oxidative activity and macrophage activation in expression of immunity to malaria.
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重新评估脾白细胞氧化活性和巨噬细胞活化在疟疾免疫表达中的作用。
DOI：
10.1128/iai.57.12.3677-3682.1989
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发表时间：
1989
影响因子：
3.1
通讯作者：
Weidanz,WP
中科院分区：
文献类型：
--
作者：
Cavacini,LA;Guidotti,M;Parke,LA;Melancon-Kaplan,J;Weidanz,WP
在急性恰鲍迪疟原虫疟疾期间，检查了脾白细胞氧化活性和巨噬细胞活化在保护性免疫发展中的作用。比较受感染的 BALB/c 和 P/J 小鼠的脾脏白细胞氧化活性；已知后者患有巨噬细胞功能缺陷。发现受感染的 BALB/c 小鼠的脾脏白细胞在佛波醇肉豆蔻酸酯乙酸酯刺激的化学发光和超氧阴离子产生方面显着增加，而来自受感染的 P/J 小鼠的脾白细胞的反应仅略有提高。受感染的 BALB/c 小鼠的脾白细胞中过氧化氢的释放略有增加，但在受感染的 P/J 小鼠的脾白细胞中基本保持不变。根据杀肿瘤活性的测量来评估巨噬细胞功能。未感染的 BALB/c 小鼠的脾巨噬细胞对 L929 靶细胞表现出显着的杀肿瘤活性。由于感染期间脾脏肿大，在受感染的 BALB/c 小鼠中，按每个脾脏计算的杀肿瘤活性进一步增加。相比之下，无论是否感染，P/J 小鼠脾脏巨噬细胞的杀肿瘤活性都很小。尽管存在这些差异，两种小鼠品系都出现了疟疾感染，并在 16 天内消退。因此，虽然脾白细胞产生活性氧自由基和巨噬细胞活化现象传统上与疟疾感染的解决有关，但本研究未能建立这些参数与 P. chabaudi adami 血液阶段感染的保护性免疫发展之间的相关性。
The role of splenic leukocyte oxidative activity and macrophage activation in the development of protective immunity was examined during acute Plasmodium chabaudi adami malaria. Splenic leukocyte oxidative activity was compared in infected BALB/c and P/J mice; the latter are known to suffer from defects in macrophage function. Phorbol myristate acetate-stimulated chemiluminescence and superoxide anion production by splenic leukocytes from infected BALB/c mice were found to be increased dramatically, while the response of splenic leukocytes from infected P/J mice was elevated only minimally. Hydrogen peroxide release was slightly increased in splenic leukocytes from infected BALB/c mice but remained essentially unchanged in those from infected P/J mice. Macrophage function was assessed on the basis of measurements of tumoricidal activity. Splenic macrophages from uninfected BALB/c mice displayed significant tumoricidal activity against L929 target cells. As a result of splenomegaly during infection, tumoricidal activity, when calculated on a per-spleen basis, was increased further in infected BALB/c mice. In contrast, the tumoricidal activity of splenic macrophages from P/J mice was minimal, regardless of infection. Despite these differences, both strains of mice developed malarial infections that resolved within 16 days. Thus, while the production of reactive oxygen radicals by splenic leukocytes and the phenomenon of macrophage activation have traditionally been associated with the resolution of malarial infection, this study failed to establish a correlation between these parameters and the development of protective immunity to blood-stage infection with P. chabaudi adami.