Fluorescence Intensity Calibration Using the Raman Scatter Peak of Water
Fluorescence Intensity Calibration Using the Raman Scatter Peak of Water
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DOI：
10.1366/000370209788964548
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发表时间：
2009-08-01
影响因子：
3.5
通讯作者：
Stedmon, C. A.
中科院分区：
文献类型：
--
作者：
Lawaetz, A. J.;Stedmon, C. A.
从不同的荧光光谱仪或通过相同的光谱仪但具有不同的仪器设置获得的重复样品的荧光数据可能具有很大的强度差异。为了比较这些数据，必须应用强度校准。在这里，我们解释一个简单的校准方法，荧光强度只使用水拉曼峰的积分面积。通过应用这种方法从三个不同的仪器的数据，我们表明，它是可能的，以消除仪器依赖的强度因子，我们提出了一个统一的尺度上的拉曼单位的结果。该方法是一种快速、简单的方法，适用于常规测量，不需要危险化学品。
Fluorescence data of replicate samples obtained from different fluorescence spectrometers or by the same spectrometer but with different instrument settings can have great intensity differences. In order to compare such data an intensity calibration must be applied. Here we explain a simple calibration method for fluorescence intensity using only the integrated area of a water Raman peak. By applying this method to data from three different instruments, we show that it is possible to remove instrument-dependent intensity factors, and we present results on a unified scale of Raman units. The method presented is a rapid and simple approach suitable for routine measurements with no need for hazardous chemicals.