Comparative Quantitative Prevalence of Mycobacteria and Functionally Abundant nidA , nahAc , and nagAc Dioxygenase Genes in Coal Tar Contaminated Sediments
Comparative Quantitative Prevalence of Mycobacteria and Functionally Abundant nidA , nahAc , and nagAc Dioxygenase Genes in Coal Tar Contaminated Sediments
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DOI：
10.1021/es070406c.s001
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发表时间：
2007
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J. E N N I F E-J.-E-N-N-I-F-E-2086297623;R. M. D E B R U Y N;R. S. C H E W N I N G, § A N D G A R-R.-S.-C-H-E-W-N-I-N-G,-§-A-N-D-G-A-R-2105437735;Y. S. S A Y L E R
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作者：
J. E N N I F E-J.-E-N-N-I-F-E-2086297623;R. M. D E B R U Y N;R. S. C H E W N I N G, § A N D G A R-R.-S.-C-H-E-W-N-I-N-G,-§-A-N-D-G-A-R-2105437735;Y. S. S A Y L E R
查塔努加溪超级基金场地受到金属、农药和煤焦油的严重污染，沉积物中含有高浓度的多环芳烃（PAH）。高分子量多环芳烃由于其在环境中的毒性和难降解性而受到关注;因此，对微生物如快速生长的分枝杆菌属，能够降解这些化合物。实时定量PCR检测针对多个双加氧酶基因，以评估生态和功能多样性的多环芳烃降解社区。这些检测试剂以结核分枝杆菌nidA、查塔努加溪中分枝杆菌分解代谢基因富集且可能与高分子量PAH生物降解功能相关的假设为特定目标，以nagAc和nahAc γ-变形菌为目标。双加氧酶基因丰度进行了定量比较，萘和芘矿化，时间和空间的多环芳烃浓度。nidA丰度为5.69 × 104 ~ 4.92 × 106拷贝/克沉积物，nagAc丰度为2.42 × 103 ~ 1.21 × 107拷贝/克沉积物，nahAc丰度为4.01 × 106拷贝/克沉积物。有一个显着更大的丰度nidA和nagAc在网站的多环芳烃浓度最高。此外，nidA和nagAc显著正相关（r = 0.76），表明携带这些基因的生物体共存。nidA和nagAc与芘矿化之间也存在正相关关系，表明这些基因可作为芘降解的生物标志物。快速生长的分枝杆菌的16 S rDNA克隆文库表明，人口是非常多样的，可能起着重要的作用，从查塔努加溪的高分子量多环芳烃衰减。
The Chattanooga Creek Superfund site is heavily contaminated with metals, pesticides, and coal tar with sediments exhibiting high concentrations of polycyclic aromatic hydrocarbons (PAHs). High molecular weight PAHs are of concern because of their toxicity and recalcitrance in the environment; as such, there is great interest in microbes, such as fast-growing Mycobacterium spp., capable of degradation of these compounds. Real-time quantitative PCR assays were developed targeting multiple dioxygenase genes to assess the ecology and functional diversity of PAHdegrading communities. These assays target the Mycobacterium nidA, â-proteobacteria nagAc, and γ-proteobacteria nahAc with the specific goal of testing the hypothesis that Mycobacteria catabolic genes are enriched and may be functionally associated with high molecular weight PAH biodegradation in Chattanooga Creek. Dioxygenase gene abundances were quantitatively compared to naphthalene and pyrene mineralization, and temporal and spatial PAH concentrations. nidA abundances ranged from 5.69 × 104 to 4.92 × 106 copies per gram sediment; nagAc from 2.42 × 103 to 1.21 × 107, and nahAc from below detection to 4.01 × 106 copies per gram sediment. There was a significantly greater abundance of nidA and nagAc at sites with the greatest concentrations of PAHs. In addition, nidA and nagAc were significantly positively correlated (r ) 0.76), indicating a coexistence of organisms carrying these genes. A positive relationship was also observed between nidA and nagAc and pyrene mineralization indicating that these genes serve as biomarkers for pyrene degradation. A 16S rDNA clone library of fastgrowing Mycobacteria indicated that the population is very diverse and likely plays an important role in attenuation of high molecular weight PAHs from Chattanooga Creek.