Additive effects of ciliary neurotrophic factor and testosterone on motoneuron survival; Differential effects on motoneuron size and muscle morphology
Additive effects of ciliary neurotrophic factor and testosterone on motoneuron survival; Differential effects on motoneuron size and muscle morphology
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DOI：
10.1006/exnr.2000.7475
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发表时间：
2000-10-01
影响因子：
5.3
通讯作者：
Forger, NG
中科院分区：
文献类型：
--
作者：
Varela, CR;Bengston, L;Forger, NG
替吉奥和睫状神经营养因子（CNTF）各自增强新生大鼠球海绵体肌脊髓核（SNB）中运动神经元的存活。在这里，我们直接比较CNTF和睾酮，单独和组合，对SNB运动神经元数量，SNB细胞的大小和形态的提肛肌（LA）的目标肌肉的影响。从出生后第1天到第6天（P1-P6），每天用重组人CNTF（hCNTF）、丙酸睾酮（TP）、hCNTF和TP两者或两者都不给药。在P7时评估治疗效果。TP和hCNTF各自增加SNB运动神经元的数量，并且达到相似的程度。与单独接受hCNTF或TP治疗的女性相比，hCNTF和TP治疗的女性SNB细胞显著更多。从P1到P6给予TP也增加了P7时SNB运动神经元的大小。与此相反，hcNTF单独没有显着影响SNB细胞的大小，和hCNTF与TP联合拮抗TP对运动神经元大小的影响。TP也增加了LA肌纤维的数量和LA纤维的大小，而hCNTF在这项研究中没有显着影响LA肌肉形态。免疫组织化学证实，几乎所有的SNB运动神经元的男性和女性表达CNTF α受体（CNTFR α）之间的胚胎第20天和出生后第6天。因此，TP和hCNTF对SNB运动神经元存活的影响是相加的，运动神经元存活的增加与hCNTF治疗动物靶肌肉形态学的变化无关。SNB运动神经元在围产期表达CNTFR α，因此是hCNTF作用的潜在直接位点。(C)北京大学出版社.
Testosterone and ciliary neurotrophic factor (CNTF) each enhance motoneuron survival in the spinal nucleus of the bulbocavernosus (SNB) of newborn rats. Here we directly compared the effects of CNTF and testosterone, alone and in combination, on SNB motoneuron number, SNB cell size, and morphology of the levator ani (LA) target muscle. Female rat pups were treated daily from postnatal day 1 through 6 (P1-P6) with recombinant human CNTF (hCNTF), testosterone propionate (TP), both hCNTF and TP, or neither. Effects of treatment were assessed on P7. TP and hCNTF each increased the number of SNB motoneurons and did so to a similar degree. Females treated with both hCNTF and TP had significantly more SNB cells than those receiving either hCNTF or TP alone. TP administered from P1 to P6 also increased SNB motoneuron size on P7. In contrast, hcNTF alone did not significantly affect SNB cell size, and hCNTF in combination with TP antagonized the effect of TP on motoneuron size. TP also increased LA muscle fiber number and LA fiber size, whereas hCNTF did not significantly influence LA muscle morphology in this study. Immunohistochemistry established that virtually all SNB motoneurons of both males and females express the CNTF alpha receptor (CNTFR alpha) between embryonic day 20 and postnatal day 6. Thus, effects of TP and hCNTF on SNB motoneuron survival were additive, and increases in motoneuron survival were dissociated from changes in target muscle morphology in hCNTF-treated animals. SNB motoneurons express CNTFR alpha perinatally and are therefore potential direct sites of hCNTF action. (C) 2000 Academic Press.