Targeted discovery of quantitative trait loci for resistance to northern leaf blight and other diseases of maize
Targeted discovery of quantitative trait loci for resistance to northern leaf blight and other diseases of maize
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DOI：
10.1007/s00122-011-1585-9
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发表时间：
2011-07-01
影响因子：
5.4
通讯作者：
Nelson, Rebecca
中科院分区：
文献类型：
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作者：
Chung, Chia-Lin;Poland, Jesse;Nelson, Rebecca
利用异源自交系(HIF)定位QTL定位和近等基因系(NIL)选育，以获取玉米抗病相关基因座上的差异等位基因。根据已知的热带玉米品系CML52和DK888对多种病害的抗性，选择它们作为等位基因供体。根据玉米病害QTL的共识图谱，对与多重抗病(MDR)相关的染色体区域(n=39)进行了定位。我们为目标基因座产生了分离的HIF，但在类似于基因组97%的地方是等基因的。为了验证MDR热点的CML52和DK888等位基因具有广谱抗性的假设，对HIF和衍生的近等位基因对大叶枯病(NLB)、南方叶枯病(SLB)、灰斑病(GLS)、炭疽病(ALB)、炭疽病(ASR)、普通锈病、普通黑穗病和斯图尔特枯萎病(Stewart‘s Wilt)的抗性进行了检测。共检测到4个NLBQTL、2个ASR QTL和1个Stewart枯萎病QTL。同时，对来自B73×CML52的196个重组自交系(RIL)群体进行了对NLB、GLS、SLB和ASR的抗性鉴定。所定位的QTL(NLB4个，SLB 5个，GLS 2个，ASR 2个)大多与利用NILs找到的QTL相对应。结合基于HIF和RIL的分析，我们发现了两个疾病QTL，在这些QTL上，CML52等位基因对多种疾病有利。位于1.06-1.07的QTL定位于Bin 1.06-1.07，定位于Bin 1.06-1.07的QTL定位于Bin 1.06-1.07，定位于Bin 1.06-1.07的QTL定位于Bin 6.05，定位于Bin 1.06-1.07。
To capture diverse alleles at a set of loci associated with disease resistance in maize, heterogeneous inbred family (HIF) analysis was applied for targeted QTL mapping and near-isogenic line (NIL) development. Tropical maize lines CML52 and DK888 were chosen as donors of alleles based on their known resistance to multiple diseases. Chromosomal regions ("bins"; n = 39) associated with multiple disease resistance (MDR) were targeted based on a consensus map of disease QTLs in maize. We generated HIFs segregating for the targeted loci but isogenic at similar to 97% of the genome. To test the hypothesis that CML52 and DK888 alleles at MDR hotspots condition broad-spectrum resistance, HIFs and derived NILs were tested for resistance to northern leaf blight (NLB), southern leaf blight (SLB), gray leaf spot (GLS), anthracnose leaf blight (ALB), anthracnose stalk rot (ASR), common rust, common smut, and Stewart's wilt. Four NLB QTLs, two ASR QTLs, and one Stewart's wilt QTL were identified. In parallel, a population of 196 recombinant inbred lines (RILs) derived from B73 x CML52 was evaluated for resistance to NLB, GLS, SLB, and ASR. The QTLs mapped (four for NLB, five for SLB, two for GLS, and two for ASR) mostly corresponded to those found using the NILs. Combining HIF- and RIL-based analyses, we discovered two disease QTLs at which CML52 alleles were favorable for more than one disease. A QTL in bin 1.06-1.07 conferred resistance to NLB and Stewart's wilt, and a QTL in 6.05 conferred resistance to NLB and ASR.