Effect of enzyme I of the bacterial phosphoenolpyruvate: sugar phosphotransferase system (PTS) on virulence in a murine model
Effect of enzyme I of the bacterial phosphoenolpyruvate: sugar phosphotransferase system (PTS) on virulence in a murine model
复制标题
DOI：
10.1099/mic.0.26406-0
复制
发表时间：
2003-09-01
期刊：
影响因子：
2.8
通讯作者：
Mukhija, S
中科院分区：
文献类型：
--
作者：
Kok, M;Bron, G;Mukhija, S
磷酸烯醇丙酮酸：糖磷酸转移酶系统（PTS）催化糖和己糖醇的易位，伴随着糖和己糖醇的磷酸化，并且它响应于碳水化合物的可用性调节代谢。PTS形成能量和信号转导之间的界面，并且其抑制可能具有多效性效应。它存在于大约三分之一的具有完全测序的基因组的细菌中，包括许多常见的病原体，但不存在于真核生物中。酶I（ptsl）是趋异蛋白磷酸化级联的第一组分。在鼠伤寒沙门氏菌、金黄色葡萄球菌和流感嗜血杆菌中构建pts1缺失，并在腹膜内小鼠模型中表征突变体的毒力。Sal的对数（衰减）值分别为2.3、1.4和0.9。鼠伤寒沙门氏菌Sta.和H.流感病毒PTSL突变体。衰减的程度与相应PTS的复杂性相关，其包括Sal中的大约40个分量。鼠伤寒沙门氏菌5株，而H.流感。
The phosphoenolpyruvate :sugar phosphotransferase system (PTS) catalyses translocation with concomitant phosphorylation of sugars and hexitols and it regulates metabolism in response to the availability of carbohydrates. The PTS forms an interface between energy and signal transduction and its inhibition is likely to have pleiotropic effects. It is present in about one-third of bacteria with fully sequenced genomes, including many common pathogens, but does not occur in eukaryotes. Enzyme I (ptsl) is the first component of the divergent protein phosphorylation cascade. ptsl deletions were constructed in Salmonella typhimurium, Staphylococcus aureus, and Haemophilus, influenzae and virulence of the mutants was characterized in an intraperitoneal mouse model. The log(attenuation) values were 2.3, 1.4 and 0.9 for the Sal. typhimurium, Sta. aureus and H. influenzae ptsl mutants, respectively. The degree of attenuation is correlated with the complexity of the respective PTS, which comprises approximately 40 components in Sal. typhimurium, but only 5 in H. influenzae.