SEEDING ARTERIAL PROSTHESES WITH VASCULAR ENDOTHELIUM - NATURE OF THE LINING
SEEDING ARTERIAL PROSTHESES WITH VASCULAR ENDOTHELIUM - NATURE OF THE LINING
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DOI：
10.1097/00000658-197907000-00019
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发表时间：
1979-01-01
影响因子：
9
通讯作者：
GLOVER, J
中科院分区：
文献类型：
--
作者：
HERRING, MB;DILLEY, R;GLOVER, J
植入自体血管内皮的动脉假体具有组织良好的细胞内衬。为了确定衬里细胞的性质，6只动物用涤纶假体置换肾下主动脉。在3个移植物的制备过程中，用钢丝棉拭子从隐静脉刮取内皮，悬浮在冷却的Sack溶液中，并与用于预缝合移植物的血液混合。从3个对照移植物中省略该悬浮液。6周后，取出移植物，冲洗并检查。荧光因子VII相关抗原（F VIII-RA）强烈染色衬里细胞。银硝酸盐Hautchen和EM制剂显示血管内皮特征性的衬里图案。内皮细胞特异性的韦伯-帕拉德体被确定在衬里细胞的细胞质。Masson三色染色显示接种织物内的胶原蛋白相对贫乏的结缔组织。透射电子显微镜显示血管平滑肌细胞之间的接种移植织物和衬里细胞。血管，从外囊产生，穿透织物，供应种子移植物的内囊。显然，植入犬动脉假体的内衬细胞是由血管平滑肌细胞支持的真正的血管内皮，内衬含有最少的结缔组织，并发育血管。未接种的对照移植物缺乏这些特征。
Arterial prostheses seeded with autogenous vascular endothelium have a well-organized, cellular, inner lining. To determine the nature of the lining cells, 6 animals underwent replacement of the infrarenal aorta with Dacron prostheses. During the preparation of 3 grafts, endothelium was scraped from the saphenous vein with a steel wool pledget, suspended in chilled Sack''s solution and mixed with blood used to preclot the graft. This suspension was omitted from the 3 control grafts. After 6 wk, the grafts were removed, rinsed and examined. Fluorescent Factor VII related antigen (F VIII-RA) strongly stained the lining cells. Silver nitrate Hautchen and EM preparations showed a lining pattern characteristic of vascular endothelium. Endothelial cell-specific Weibel-Palade bodies were identified in the lining cell cytoplasm. Masson''s trichrome staining revealed a relatively collagen-poor connective tissue within the seeded fabric. Transmission electron microscopy disclosed vascular smooth muscle cells between the seeded graft fabric and the lining cells. Vasa vasorum, arising from the outer capsule, penetrated the fabric to supply the inner capsules of the seeded grafts. Apparently the cells lining seeded canine arterial prostheses are true vascular endothelium supported by vascular smooth muscle cells, the lining contains minimal connective tissue, and vasa vasorum develop. Unseeded control grafts lacked these features.