Cesium ion liquid secondary ion mass spectrometry of membrane-bound glycoproteins: structural and topological considerations of acetylcholine receptor from Torpedo californica.
Cesium ion liquid secondary ion mass spectrometry of membrane-bound glycoproteins: structural and topological considerations of acetylcholine receptor from Torpedo californica.
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膜结合糖蛋白的铯离子液体二次离子质谱：加州鱼雷乙酰胆碱受体的结构和拓扑考虑。
DOI：
10.1002/bms.1200160105
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Burlingame,AL
中科院分区：
文献类型：
--
作者：
Poulter,L;Earnest,JP;Stroud,RM;Burlingame,AL
We report mass mapping of a large (270 kD) multisubunit membrane bound glycoprotein, nicotinic acetylcholine receptor fromTorpedo californica, using enzymic digests of the affinity purified whole receptor and cesium ion liquid secondary ion mass spectrometry. Peptides, glycopeptides and derivatized N‐linked oligosaccharides were isolated by HPLC and identified by LSIMS. We have shown that mass spectrometric sensitivity is improved a hundred‐fold through use of computer‐controlled mass window stepping of an electro‐optical multichannel array detection system on a LSIMS double focusing mass spectrometer. This new method permitted determination of the complete fragmentation pattern of Man8N2‐ABEE using only 5 picomoles of sample.
