c-fos expression as a marker of central cardiovascular neurons.
c-fos expression as a marker of central cardiovascular neurons.
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DOI：
10.1159/000109431
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发表时间：
1995-05
期刊：
影响因子：
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通讯作者：
N. Dun;S. L. Dun;E. Shen;H. Tang;R. Huang;T. Chiu
中科院分区：
文献类型：
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作者：
N. Dun;S. L. Dun;E. Shen;H. Tang;R. Huang;T. Chiu
Fos的免疫组织化学检测，即早期基因c-fos的蛋白产物，作为中枢神经元对血压/血容量变化敏感的功能标志物进行了评价。控制性出血和注入膨胀剂硝普钠或肼苯哒嗪诱导几组显著的中枢神经元出现Fos免疫反应性（Fos-IR）：梨状皮质、终纹床核、Calleja岛、穹窿下器、杏仁核中央核、臂旁核、视上核和室旁核、脑桥A5、蓝斑、延髓腹外侧区、脊髓中的孤束核、最后区和中间外侧细胞柱。血压升高苯肾上腺素输液引起的外观Fos-IR在少数几组神经元：床核的终纹，中央核的杏仁核，臂旁核，孤束核和最后区。Fos神经元在肥胖与高血压动物中的差异分布强调了Fos作为代谢标志物在识别响应特定心血管挑战的神经元网络中的潜在应用。此外，含Fos神经元的递质表型的同时表征提供了一个额外的优势，这种方法比其他传统的束跟踪技术。
Immunohistochemical detection of Fos, the protein product of the immediate-early gene c-fos, was evaluated as a functional marker of central neurons sensitive to a change of blood pressure/blood volume. Controlled hemorrhage and infusion of the hypotensive agent nitroprusside or hydralazine induced the appearance of Fos-immunoreactivity (Fos-IR) in several prominent groups of central neurons: the piriform cortex, bed nucleus of the stria terminalis, islands of Calleja, subfornical organ, central nucleus of the amygdala, parabrachial nucleus, supraoptic and paraventricular nuclei, pontine A5, locus ceruleus, ventrolateral medulla, the nucleus of the solitary tract, area postrema, and intermediolateral cell column in the spinal cord. Elevation of blood pressure by infusion of phenylephrine caused the appearance of Fos-IR in fewer groups of neurons: the bed nucleus of the stria terminalis, central nucleus of the amygdala, parabrachial nucleus, the nucleus of the solitary tract and area postrema. The differential distribution of Fos neurons in hypotensive versus hypertensive animals underscores the potential application of Fos as a metabolic marker in identifying a network of neurons responding to a specific cardiovascular challenge. Further, simultaneous characterization of the transmitter phenotype of Fos-containing neurons offers an additional advantage of this method over other conventional tract-tracing techniques.