Hydrodynamic slip on DNA observed by optical tweezers-controlled translocation experiments with solid-state and lipid-coated nanopores.
Hydrodynamic slip on DNA observed by optical tweezers-controlled translocation experiments with solid-state and lipid-coated nanopores.
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DOI：
10.1021/nl501909t
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发表时间：
2014-06
期刊：
影响因子：
10.8
通讯作者：
Lukas Galla;Andreas J Meyer;A. Spiering;A. Sischka;Michael Mayer;A. Hall;P. Reimann;D. Anselmetti
中科院分区：
文献类型：
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作者：
Lukas Galla;Andreas J Meyer;A. Spiering;A. Sischka;Michael Mayer;A. Hall;P. Reimann;D. Anselmetti
我们使用光钳研究了直径在6到70 nm之间的氮化硅纳米孔内单个dsDNA分子的穿透力，以及脂膜固体纳米孔内的穿线力。我们观察到减小纳米孔尺寸的穿线力显著增加，这可以归因于与电泳相反的电渗流(EOF)的显著减少。此外，我们还表明，还可以通过在纳米孔壁上涂覆一层电中性脂质双层来降低EOF，从而使穿线力增加85%。所有的实验结果都可以用一个定量的理论模型来描述，该模型考虑了DNA表面的流体动力滑移效应，滑移长度为0.5 nm。
We use optical tweezers to investigate the threading force on a single dsDNA molecule inside silicon-nitride nanopores between 6 and 70 nm in diameter, as well as lipid-coated solid-state nanopores. We observe a strong increase of the threading force for decreasing nanopore size that can be attributed to a significant reduction in the electroosmotic flow (EOF), which opposes the electrophoresis. Additionally, we show that the EOF can also be reduced by coating the nanopore wall with an electrically neutral lipid bilayer, resulting in an 85% increase in threading force. All experimental findings can be described by a quantitative theoretical model that incorporates a hydrodynamic slip effect on the DNA surface with a slip length of 0.5 nm.