Spatiotemporally controlled contraction of micropatterned skeletal muscle cells on a hydrogel sheet
Spatiotemporally controlled contraction of micropatterned skeletal muscle cells on a hydrogel sheet
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DOI：
10.1039/c01c00364f
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发表时间：
2011-01-01
期刊：
影响因子：
6.1
通讯作者：
Nishizawa, Matsuhiko
中科院分区：
文献类型：
--
作者：
Nagamine, Kuniaki;Kawashima, Takeaki;Nishizawa, Matsuhiko
我们开发了凝胶片支持的C2 C12肌管微模式，并将其与微电极阵列芯片相结合，以提供骨骼肌细胞为基础的生物测定系统。在玻璃基质上培养的肌管线图案以100%的效率转移到纤维蛋白凝胶片的表面。凝胶上的每个肌管线图案的收缩行为通过局部电刺激单独控制，所述局部电刺激使用先前用电聚合的聚（3，4-亚乙基二氧噻吩）（PEDOT）改性的微电极阵列。我们成功地证明了荧光成像的收缩诱导易位的葡萄糖转运蛋白，GLUT 4，从细胞内囊泡的肌管质膜。该装置适用于骨骼肌细胞中收缩诱导的代谢改变的生物测定。
We have developed gel sheet-supported C2C12 myotube micropatterns and combined them with a microelectrode array chip to afford a skeletal muscle cell-based bioassay system. Myotube line patterns cultured on a glass substrate were transferred with 100% efficiency to the surface of fibrin gel sheets. The contractile behavior of each myotube line pattern on the gel was individually controlled by localized electrical stimulation using microelectrode arrays that had been previously modified with electropolymerized poly(3,4-ethylenedioxythiophene) (PEDOT). We successfully demonstrated fluorescent imaging of the contraction-induced translocation of the glucose transporter, GLUT4, from intracellular vesicles to the plasma membrane of the myotubes. This device is applicable for the bioassay of contraction-induced metabolic alterations in a skeletal muscle cell.