Anticoagulant fucoidan fractions from Fucus vesiculosus induce platelet activation in vitro
Anticoagulant fucoidan fractions from Fucus vesiculosus induce platelet activation in vitro
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DOI：
10.1016/s0049-3848(97)00037-6
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发表时间：
1997-03-15
影响因子：
7.5
通讯作者：
Beress, L
中科院分区：
文献类型：
--
作者：
Durig, J;Bruhn, T;Beress, L
Anticoagulant fucoidan fractions of different molecular weight and sulfate content were prepared and investigated for their effects on platelet function in vitro. The fucoidan fractions were incubated with human platelet rich plasma (PRP) at concentrations of 5, 10 and 50 mu g/ml. Platelet activation was subsequently studied by a standard aggregation assay and flow cytometric determination of the activation dependent platelet-surface markers CD62p (P-selectin, GMP-140) and CD63 (GP53). All fucoidan fractions induced irreversible platelet aggregation in a dose-dependent manner. Comparing fractions of identical molecular weight (100 kDa) the low sulfate content fucoidan FF5 (S=7,6 %) exerted a significantly greater effect than the highly sulfated fucoidan FF7 (S= 10,2 %) over the whole concentration range (n=5, P
