Bacterial invasion and transcytosis in transfected human brain microvascular endothelial cells
Bacterial invasion and transcytosis in transfected human brain microvascular endothelial cells
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DOI：
10.1006/mpat.2000.0406
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发表时间：
2001-01-01
影响因子：
3.8
通讯作者：
Kim, KS
中科院分区：
文献类型：
--
作者：
Stins, MF;Badger, J;Kim, KS
大多数新生儿细菌性脑膜炎病例是由于血行播散而发展的，但目前尚不清楚循环细菌如何穿过血脑屏障。由于缺乏可靠的人类血脑屏障模型，回答这些问题的努力受到了阻碍。分离人脑微血管内皮细胞（HBMEC），用含有猴病毒40大T抗原（SV 40-LT）的pBR 322质粒转染HBMEC。转染的HBMEC表现出与原代HBMEC相似的脑内皮细胞特征，即γ谷氨酰转肽酶和高跨内皮电阻。土栖大肠杆菌和柠檬酸杆菌属，两种重要的革兰氏阴性杆菌引起新生儿脑膜炎，被发现转胞质跨越原代和转染的HBMEC，而不影响单层的完整性。此外，E. coli和C.弗氏杆菌如先前用原代HBMEC所示侵入转染的HBMEC。我们的结论是E. coli和C.弗氏杆菌能够侵入和转胞吞HBMEC，并且这些细菌-HBMEC相互作用在原代和转染的HBMEC之间是相似的。因此，我们的转染HBMEC应该是有用的研究中枢神经系统感染的发病机制。(C)北京：科学出版社.
Most cases of neonatal bacterial meningitis develop as a result of a hematogenous spread, but it is not clear how circulating bacteria cross the blood-brain barrier. Attempts to answer these questions have been hampered by the lack of a reliable model of the human blood-brain barrier. Human brain microvascular endothelial cells (HBMEC) were isolated and transfected with a pBR322 based plasmid containing simian virus 40 large T antigen (SV40-LT). The transfected HBMEC exhibited similar brain endothelial cell characteristics as the primary HBMEC, i.e. gamma glutamyl transpeptidase and a high transendothelial electrical resistance. Escherischia coli and Citrobacter spp, two important Gram-negative bacilli causing neonatal meningitis, were found to transcytose across primary and transfected HBMEC, without affecting the integrity of the monolayer. In addition, E. coli and C. freundii invaded transfected HBMEC as shown previously with primary HBMEC. We conclude that E. coli and C. freundii are able to invade and transcytose HBMEC and these bacterial-HBMEC interactions are similar between primary and transfected HBMEC. Therefore, our transfected HBMEC should be useful for studying pathogenesis of CNS infections. (C) 2001 Academic Press.