Effect of pomegranate peel polyphenol gel on cutaneous wound healing in alloxan-induced diabetic rats
Effect of pomegranate peel polyphenol gel on cutaneous wound healing in alloxan-induced diabetic rats
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DOI：
10.3760/cma.j.issn.0366-6999.20122728
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发表时间：
2013-05-05
影响因子：
6.1
通讯作者：
Wang Xin-chun
中科院分区：
文献类型：
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作者：
Yan Huan;Peng Ke-jun;Wang Xin-chun
研究背景石榴（punica granatum）为石榴科石榴属植物，其果皮具有抑肠、止血、杀虫等功效，是一种常用的中药。据报道，菠萝皮具有伤口愈合特性，这主要归因于其多酚提取物。为探讨石榴皮多酚（PPP）凝胶对糖尿病大鼠皮肤创面愈合的影响，采用四氧嘧啶（Alloxan）诱导的糖尿病大鼠背部正中切口，每天给予PPP凝胶（多酚质量分数为30%）治疗。分别于伤后第4、7、14、21天处死大鼠，观察伤口愈合率、组织学特征;检测羟脯氨酸含量、一氧化氮（NO）生成量、一氧化氮合酶（NOS）活性及转化生长因子β 1（TGF β 1）表达结果PPP凝胶应用于糖尿病大鼠创面后，创面愈合时间明显缩短。组织学检查显示PPP凝胶能够增加糖尿病大鼠伤口区域中的成纤维细胞浸润、胶原再生、血管形成和上皮形成。PPP凝胶治疗后糖尿病大鼠创面组织中羟脯氨酸含量、NO含量、NOS活性增加，TGF-β 1、VEGF、EGF表达增加。结论PPP凝胶治疗糖尿病创面可能是一种有益的治疗方法。
Background Pomegranate (punica granatum) belongs to the family Punicaceae, and its peel has been used as a traditional Chinese medicine because of its efficacy in restraining intestine, promoting hemostasis, and killing parasites. Pomegranate peel has been reported to possess wound-healing properties which are mainly attributed to its polyphenol extracts. The purpose of this study was to investigate the effect of pomegranate peel polyphenols (PPP) gel on cutaneous wound healing in diabetic rats.Methods Alloxan-induced diabetic rats were given incisional wounds on each side of the mid-back and then treated daily with PPP gel (polyphenol mass fraction =30%) post-wounding. Rats were sacrificed on days 4, 7, 14, and 21 post-wounding to assess the rates of wound closure, histological characteristics; and to detect the contents of hydroxyproline, production of nitric oxide (NO), and activities of NO synthase (NOS), as well as the expressions of transforming growth factor-beta 1 (TGF-beta 1), vascular endothelial growth factor (VEGF), and epidermal growth factor (EGF) in wound tissue.Results Wound closure was significantly shortened when PPP gel was applied to the wounds of diabetic rats. Histological examination showed the ability of PPP gel to increase fibroblast infiltration, collagen regeneration, vascularization, and epithelialization in the wound area of diabetic rats. In addition, PPP gel-treated diabetic rats showed increased contents of hydroxyproline, production of NO, and activities of NOS and increased expressions of TGF-beta 1, VEGF, and EGF in wound tissues.Conclusion PPP gel may be a beneficial method for treating wound disorders associated with diabetes.