The zebrafish klf gene family
The zebrafish klf gene family
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DOI：
10.1182/blood.v98.6.1792
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发表时间：
2001-09-15
期刊：
影响因子：
20.3
通讯作者：
Zon, LI
中科院分区：
文献类型：
--
作者：
Oates, AC;Pratt, SJ;Zon, LI
Kroppel-like factor(KLF)家族基因编码转录调节蛋白，在哺乳动物多种细胞的分化中发挥作用。例如，创始成员KLF1(也称为EKLF)是哺乳动物正常产生珠蛋白所必需的。从斑马鱼Danio rerio中分离到5个新的KLF基因，并对其产物的结构、遗传图谱的位置及其在斑马鱼发育过程中的表达进行了研究。发现了与哺乳动物KLF2和KLF4密切相关的三个基因，以及哺乳动物KLF12的同源基因。第五个基因显然是哺乳动物基因组中缺失的，与KLF1和KLF2密切相关，也被发现。分析表明，在这些蛋白质的N-末端存在新的保守结构域。发育中的表达模式表明，这些斑马鱼基因在不同的过程中可能发挥作用，包括造血、血管功能、鳍和表皮发育。这些研究表明，斑马鱼的基因与它们的哺乳动物同源物具有高度的功能保守性。这些发现进一步加深了对KLF基因在脊椎动物发育中的理解，并表明Kroppel样因子基因家族在造血中的古老作用。(C)2001年，由美国血液病学会提供。
The Kroppel-like factor (KLF) family of genes encodes transcriptional regulatory proteins that play roles in differentiation of a diverse set of cells in mammals. For instance, the founding member KLF1 (also known as EKLF) is required for normal globin production in mammals. Five new KLF genes have been isolated from the zebrafish, Danio rerio, and the structure of their products, their genetic map positions, and their expression during development of the zebrafish have been characterized. Three genes closely related to mammalian KLF2 and KLF4 were found, as was an ortholog of mammalian KLF12. A fifth gene, apparently missing from the genome of mammals and closely related to KLF1 and KLF2, was also identified. Analysis demonstrated the existence of novel conserved domains in the N-termini of these proteins. Developmental expression patterns suggest potential roles for these zebrafish genes in diverse processes, including hematopoiesis, blood vessel function, and fin and epidermal development. The studies imply a high degree of functional conservation of the zebrafish genes with their mammalian homologs. These findings further the understanding of the KLF genes in vertebrate development and indicate an ancient role in hematopoiesis for the Kroppel-like factor gene family. (C) 2001 by The American Society of Hematology.