Preventive effects of nucleoprotein supplementation combined with intermittent loading on capillary regression induced by hindlimb unloading in rat soleus muscle.
Preventive effects of nucleoprotein supplementation combined with intermittent loading on capillary regression induced by hindlimb unloading in rat soleus muscle.
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DOI：
10.14814/phy2.13134
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发表时间：
2017-02
影响因子：
2.5
通讯作者：
Fujino H
中科院分区：
文献类型：
--
作者：
Hirayama Y;Nakanishi R;Maeshige N;Fujino H
缺乏体力活动会导致骨骼肌的肌肉萎缩和毛细血管退化。后肢去负荷时间歇性负荷减轻了骨骼肌的萎缩，但不抑制骨骼肌毛细血管的消退。核蛋白具有抗氧化能力，可防止毛细血管退化。因此，我们评估了间歇性负荷与核蛋白补充对后肢卸载引起的毛细血管消退的联合作用。将大鼠分为5组：对照组（CON）、7天后肢去负荷组（HU）、HU+核蛋白补充组（HU + NP）、HU间歇负荷组（HU + IL）和HU间歇负荷+核蛋白补充组（HU + IL + NP）。7天HU导致毛细血管数与纤维数（C/F）比值降低，伴有比目鱼肌中胎肝激酶-1（Flk-1）（一种促血管生成因子）和血小板反应蛋白-1（TSP-1）（一种抗血管生成因子）的废用相关变化。此外，柠檬酸合酶（CS）活性降低，超氧化物歧化酶（SOD）-2蛋白水平升高。无论是补充核蛋白，也不间歇性负荷防止C/F比的下降，而补充核蛋白与间歇性负荷相结合，防止卸载过程中毛细血管的回归。此外，Flk-1、TSP-1和SOD-2蛋白水平以及CS活性维持在对照水平。这些结果表明，核蛋白补充结合间歇负荷是有效的，以防止毛细血管萎缩引起的肌肉萎缩。
Physical inactivity leads to muscle atrophy and capillary regression in the skeletal muscle. Intermittent loading during hindlimb unloading attenuates the muscle atrophy, meanwhile the capillary regression in the skeletal muscle is not suppressed. Nucleoprotein has antioxidant capacity and may prevent capillary regression. Therefore, we assessed the combined effects of intermittent loading with nucleoprotein supplementation on capillary regression induced by hindlimb unloading. Five groups of rats were assigned: control (CON), 7 days hindlimb unloading (HU), HU plus nucleoprotein supplementation (HU + NP), intermittent loading during HU (HU + IL), and intermittent loading combined with nucleoprotein supplementation during HU (HU + IL + NP). Seven days HU resulted in decrease in capillary number‐to‐fiber number (C/F) ratio accompanied with disuse‐associated changes in fetal liver kinase‐1 (Flk‐1), a proangiogenesis factor, and thrombospondin‐1 (TSP‐1), an antiangiogenesis factor, in the soleus muscle. In addition, citrate synthase (CS) activity was decreased and protein level of superoxide dismutase (SOD)‐2 was increased. Neither nucleoprotein supplementation nor intermittent loading prevented the decrease in the C/F ratio, whereas nucleoprotein supplementation combined with intermittent loading prevented the regression of capillary during unloading. Moreover, the levels of Flk‐1, TSP‐1, and SOD‐2 protein and the CS activity were maintained up to control levels. These results suggested that nucleoprotein supplementation combined with intermittent loading was effective to prevent capillary regression induced by muscle atrophy.