Simple colorimetric DNA detection based on hairpin assembly reaction and target-catalytic circuits for signal amplification
Simple colorimetric DNA detection based on hairpin assembly reaction and target-catalytic circuits for signal amplification
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基于发夹组装反应和用于信号放大的靶催化电路的简单比色 DNA 检测
DOI：
10.1016/j.ab.2012.07.009
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发表时间：
2012-10-15
影响因子：
2.9
通讯作者：
Wang, Qingyin
中科院分区：
文献类型：
--
作者：
Ma, Cuiping;Wang, Wenshuo;Wang, Qingyin
提出了一种基于发夹组装反应和靶催化DNA电路实现无酶信号放大的简单比色DNA检测策略。该方法采用了两种发夹种类（H1和H2），它们是稳定的，在没有靶标的情况下不能杂交。在靶标存在下，靶标与发夹H1杂交，打开的发夹H1与发夹H2杂交，使得靶标被置换。将H1和H2分别连接到金纳米颗粒上，使得由H1和H2形成的双链体能够用肉眼观察到。移位的靶标再次触发下一轮链交换反应以实现信号放大。该方法可以具有广泛的传感器应用，因为它是无酶的和简单的执行。皇冠版权所有（C）2012由Elsevier Inc.发布。All rights reserved.
A simple strategy of colorimetric DNA detection is presented based on a hairpin assembly reaction and target-catalytic DNA circuits to achieve enzyme-free signal amplification. The method employed two hairpin species (H1 and H2), which were stable and unable to hybridize in the absence of target. In the presence of target, the target hybridized with hairpin H1 and the opened hairpin HI hybridized with hairpin H2, allowing the target to be displaced. H1 and H2 were respectively attached to gold nanoparticles, allowing the duplex formed from H1 and H2 to be visualized with the naked eye. The displaced target again triggered the next round of strand exchange reaction to achieve signal amplification. The method may have a wide range of sensor applications because it is enzyme-free and simple to perform. Crown Copyright (C) 2012 Published by Elsevier Inc. All rights reserved.