Generation and partial characterization of melanoma sublines resistant to lymphokine activated killer (LAK) cells. Relevance to doxorubicin resistance
Generation and partial characterization of melanoma sublines resistant to lymphokine activated killer (LAK) cells. Relevance to doxorubicin resistance
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对淋巴因子激活杀伤 (LAK) 细胞具有抗性的黑色素瘤亚系的产生和部分表征。
DOI：
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发表时间：
1989
影响因子：
6.4
通讯作者：
G. Parmiani
中科院分区：
文献类型：
--
作者：
L. Rivoltini;C. Gambacorti;R. Supino;G. Parmiani
为了了解肿瘤细胞群体是否可能含有对淋巴因子激活的杀伤细胞(LAK)具有抵抗力的细胞，来自LAK敏感黑色素瘤细胞系(ME665/2)的细胞与LAK细胞共培养。免疫选择1、2、3个周期后获得3个亚系。与亲本Me 665/2相比，免疫选择(IS)亚系表现出增殖减少，对单抗(MAb)R24的反应性降低，在形态上表现出更大的分化。在IS亚系中观察到对LAK的敏感性逐渐降低，LAK敏感性降低了8倍以上。在IS亚系中也观察到补体(C)介导的裂解作用减少。由于我们之前已经证明黑色素瘤细胞的LAK敏感性可能与阿霉素(Dx)耐药有关，因此在这些株系中测试了对Dx的敏感性。与Me 665/2相比，IS亚系对Dx的敏感性增强。IS亚系与亲本Me 665/2对LAK敏感性的差异在2周内保持稳定，但此后下降并消失。这些结果表明：(1)LAK敏感的肿瘤细胞系可能含有LAK裂解显著减少的细胞亚群；(2)LAK敏感性与C介导的裂解敏感性之间也存在相关性；(3)IS亚系对Dx的敏感性增加是明显的。
To see whether a tumor cell population may contain cells resistant to lymphokine‐activated killer (LAK) lymphocytes, cells from a LAK‐sensitive melanoma line (Me 665/2) were co‐cultured with LAKs. Three sublines were obtained after 1, 2 or 3 immunoselection cycles. Immunoselected (IS) sublines show reduced proliferation, decreased reactivity to the monoclonal antibody (MAb) R24 and appeared morphologically more differentiated in comparison with the parental Me 665/2 line. A progressively reduced sensitivity to LAKs was observed in IS sublines with a more than 8‐fold reduction in LAK susceptibility. A reduced complement (C)‐mediated lysis was also observed in IS sublines. Since we have previously shown that LAK sensitivity of melanoma cells may be associated with Doxorubicin (Dx) resistance, the sensitivity to Dx was tested in these lines. An augmented sensitivity to Dx was noted in IS sublines as compared with Me 665/2. The differences in LAK susceptibility between the IS sublines and the parental Me 665/2 line remained stable for 2 weeks but declined and disappeared thereafter. These results indicate that (1) a LAK‐sensitive tumor line may contain a subpopulation of cells which are significantly less lysed by LAKs; (2) a correlation between LAK sensitivity and susceptibility to C‐mediated lysis is also present; and (3) increased sensitivity to Dx is evident in the IS sublines.
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DOI：
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发表时间：
1987-05
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
M. Herlyn;W. Clark;U. Rodeck;M. Mancianti;J. Jambrosic;H. Koprowski
通讯作者：
M. Herlyn;W. Clark;U. Rodeck;M. Mancianti;J. Jambrosic;H. Koprowski
不同大小的人类原发性黑色素瘤集落重新接种在琼脂中，具有相似的自我更新特性。
影响因子：
11.2
作者：
MeyskensJr,FL;Thomson,SP;Moon,TE
通讯作者：
Moon,TE
通过白细胞介素 2 刺激的细胞毒性淋巴细胞对已建立的小鼠肾癌进行辅助免疫治疗。
影响因子：
11.2
作者：
Salup,RR;Wiltrout,RH
通讯作者：
Wiltrout,RH
通过高剂量白细胞介素2治疗根除播散性小鼠白血病。
DOI：
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发表时间：
1986
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Thompson,JA;Peace,DJ;Klarnet,JP;Kern,DE;Greenberg,PD;Cheever,MA
通讯作者：
Cheever,MA