In vitro characterization of two different ultrasmall iron oxide particles for magnetic resonance cell tracking
In vitro characterization of two different ultrasmall iron oxide particles for magnetic resonance cell tracking
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DOI：
10.1097/00004424-200209000-00002
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发表时间：
2002-09-01
影响因子：
6.7
通讯作者：
Zimmer, C
中科院分区：
文献类型：
--
作者：
Fleige, G;Seeberger, F;Zimmer, C
理论基础和目标。比较两种超小超顺磁性氧化铁(USPIO)颗粒对巨噬细胞的细胞内标记特性和随后的磁共振成像可视化。材料和方法。将巨噬细胞株P-388D1与中性羧基葡聚糖包被的USPIO制剂(DDM 43/34/103)或酸性柠檬酸包被的USPIO(VSOP-C125)孵育。实验中改变了孵育浓度和孵育时间，并用特定的抑制剂抑制吞噬和吞噬作用。结果：VSOP-C125被细胞掺入的速度比DDM 43/34/103快得多，并且每个细胞产生的最终细胞内铁浓度显著高于DDM 43/34/103(3420对727 ng/百万细胞)。这两种制剂在磁共振成像中相对于每个细胞的铁含量显示出相似的信号减少效应。结论柠檬酸包被的USPIO颗粒VSOP-C125对巨噬细胞的磁性标记性能明显优于DDM 43/34/103包被的USPIO制剂。
RATIONALE AND OBJECTIVES. Comparison of two different ultrasmall superparamagnetic iron oxide (USPIO) particles in terms of their intracellular cell-labeling properties of macrophages and subsequent visualization by MR imaging.MATERIALS AND METHODS. Cultures containing the macrophage cell line P-388D1 were incubated with a neutral carboxydextran-coated USPIO preparation (DDM 43/34/103) or an acidic citrate-coated USPIO (VSOP-C125). Experiments were performed in which incubation concentration and duration were varied and phagocytosis and pinocytosis suppressed by specific inhibitors. In cell culture specimens iron content was measured quantitatively and signal intensities determined by in vitro MR imaging.RESULTS. VSOP-C125 is incorporated by cells much faster than DDM 43/34/103 and produces significantly higher final intracellular iron concentrations per cell (3420 vs. 727 ng/million cells). Both preparations show similar signal-reducing effects at MR imaging relative to the Fe content per cell. Intracellular USPIO has a much lower detection threshold at MR imaging (50/80 mumol/L) than extracellular USPIO in free solution (300 mumol/L).CONCLUSIONS. Citrate-coated USPIO particles VSOP-C125 appear to have more favorable properties for magnetic labeling of macrophages than the carboxydextran-coated USPIO preparation DDM 43/34/103.