RIBONUCLEOTIDE REDUCTASE M2 SUBUNIT SEQUENCES MAPPED TO FOUR DIFFERENT CHROMOSOMAL SITES IN HUMANS AND MICE FUNCTIONAL LOCUS IDENTIFIED BY ITS AMPLIFICATION IN HYDROXYUREA-RESISTANT CELL LINES
RIBONUCLEOTIDE REDUCTASE M2 SUBUNIT SEQUENCES MAPPED TO FOUR DIFFERENT CHROMOSOMAL SITES IN HUMANS AND MICE FUNCTIONAL LOCUS IDENTIFIED BY ITS AMPLIFICATION IN HYDROXYUREA-RESISTANT CELL LINES
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DOI：
10.1016/0888-7543(87)90108-x
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发表时间：
1987-01-01
期刊：
影响因子：
4.4
通讯作者：
FRANCKE U
中科院分区：
文献类型：
--
作者：
YANG-FENG T L;BARTON D E;FRANCKE U
采用种间体细胞杂交系的southern blot分析和原位杂交技术，在人和鼠染色体上确定了核糖核苷酸还原酶（RR） M2亚基cDNA同源位点。在人类基因组中，在1p31 .fwdarw处鉴定出4个携带rrm2相关序列的染色体位点。P33, 1q21 .fwdarw。Q23, 2p24 .fwdarw。和Xp11 .fwdarw。p21。在小鼠体细胞杂交研究中，在染色体4、7、12和13上发现了M2序列。通过Southern对人类抗羟基脲细胞的分析，由于基因扩增而过量产生M2，我们已经确定了扩增的限制性片段是那些映射到2号染色体的片段。为了进一步确认功能性RRM2位点的位置，我们利用Southern杂交和原位杂交技术，绘制了另外两个cDNA克隆，p5-8和S7（编码鸟氨酸脱羧酶；ODC），它们与RRM2序列在人和啮齿动物抗羟基脲细胞系中共扩增。它们的染色体图谱位置与人类2号染色体区域一致（p24 .fwdarw）。p25)，也包含四个rrm2样序列之一。由于该RRM2序列与p5-8和ODC很可能是同一扩增单元的一部分，因此RRM2结构基因可以分配到人类染色体2p24 .fwdarw上。p25。该区域与12号染色体的一个区域同源，该区域也携带许多odc样序列之一。在过量产生rrm2的小鼠细胞系中，我们发现了12号染色体特异性限制性片段的扩增。因此，我们得出结论，小鼠12号染色体携带RRM2的功能位点。
The sites of sequences homologous to a murine cDNA for ribonucleotide reductase (RR) subunit M2 were determined on human and murine chromosomes by southern blot analysis of interspecies somatic cell hybrid lines and by in situ hybridization. In the human genome, four chromosomal sites carrying RRm2-related sequences were identified at 1p31 .fwdarw. p33, 1q21 .fwdarw. q23, 2p24 .fwdarw. p25, and Xp11 .fwdarw. p21. In the mouse, M2 sequences were found on chromosomes 4, 7, 12, and 13 by somatic cell hybrid studies. By Southern analysis of human hydroxyurea-resistant cells that overproduce M2 because of gene amplification, we have identified the amplified restriction fragments as those that map to chromosome 2. To further confirm the site of the functional RRM2 locus, two other cDNA clones, p5-8 and S7 (coding for ornithine decarboxylase; ODC), which are coamplified with RRM2 sequences in human and rodent hydroxyurea-resistant cell lines, were mapped by Southern and in situ hybridization. Their chromosomal map positions coincided with the region of human chromosome 2 (p24 .fwdarw. p25) that also contains one of the four RRM2-like sequences. Since this RRM2 sequence and p5-8 and ODC are most likely part of the same amplification uinit, the RRM2 structural gene can be assigned to human chromosome 2p24 .fwdarw. p25. This region is homologous to a region of msouse chromosome 12 that also carries one of numerous ODC-like sequences. In an RRM2-overproducing mouse cell line, we found amplificaiton of the chromosome 12-specific restriction fragments. Thus, we conclude that mouse chromosome 12 carries the functional locus for RRM2.